Recombinant Drosophila melanogaster Optomotor-blind protein (bi), partial

Code CSB-YP630511DLU
MSDS
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Source Yeast
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Code CSB-EP630511DLU
MSDS
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Source E.coli
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Code CSB-EP630511DLU-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP630511DLU
MSDS
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Source Baculovirus
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Code CSB-MP630511DLU
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
bi
Uniprot No.
Alternative Names
bi; OMB; CG3578; Optomotor-blind protein; Lethal(1)optomotor-blind; L(1)omb; Protein bifid
Species
Drosophila melanogaster (Fruit fly)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Essential protein that may function as a transcription regulator. Vital for pupal development. Required for proper development of the optic lobes and wings, and abdominal pigmentation.
Gene References into Functions
  1. complementary expression of omb and Iro-C establishes the wing hinge/notum fold formation PMID: 27212024
  2. Optomotor-blind does not prevent apoptosis in the haltere but rather limits growth by repressing several Decapentaplegic and Hedgehog targets. PMID: 25793870
  3. results suggest that the primary effect of Omb is the blocking of Jak/STAT signaling by repressing transcription of upd which encodes the Jak receptor ligand Unpaired. PMID: 25781970
  4. Since loss of omb disrupts horizontal system and vertical system cell development, we assume that HS/VS ontogeny is controlled by Omb target genes. PMID: 24912380
  5. Dpp, via omb, promoted proliferation in the lateral and repressed proliferation in the medial wing disc. Omb controlled the regional proliferation rate by oppositely regulating transcription of the microRNA gene bantam in medial versus lateral wing disc. PMID: 23821035
  6. four new omb mutations were isolated and characterized. PMID: 20033428
  7. dysfunction of omb elicits hyperactivation of Hh signaling that causes an ectopic folding and local overgrowth in the wing columnar epithelium PMID: 17573067
  8. OMB exprsssion in glial cells is required for correct axonal projection across the Drosophila inner optic chiasma. PMID: 18234176

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Subcellular Location
Nucleus.
Tissue Specificity
In third-instar larvae, expressed in the brain region that will develop into optic lobes and more weakly in the thoracic part of the ventral ganglion.
Database Links

KEGG: dme:Dmel_CG3578

STRING: 7227.FBpp0303589

UniGene: Dm.1683

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