bi Antibody

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Code	CSB-PA630511XA01DLU
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Target Background

Product Details

Full Product Name

Rabbit anti-Drosophila melanogaster (Fruit fly) bi Polyclonal antibody

Uniprot No.

Q24432

Target Names

Alternative Names

bi antibody; OMB antibody; CG3578 antibody; Optomotor-blind protein antibody; Lethal(1)optomotor-blind antibody; L(1)omb antibody; Protein bifid antibody

Raised in

Rabbit

Species Reactivity

Drosophila melanogaster (Fruit fly)

Immunogen

Recombinant Drosophila melanogaster (Fruit fly) bi protein

Immunogen Species

Drosophila melanogaster (Fruit fly)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

Essential protein that may function as a transcription regulator. Vital for pupal development. Required for proper development of the optic lobes and wings, and abdominal pigmentation.

Gene References into Functions

complementary expression of omb and Iro-C establishes the wing hinge/notum fold formation PMID: 27212024
Optomotor-blind does not prevent apoptosis in the haltere but rather limits growth by repressing several Decapentaplegic and Hedgehog targets. PMID: 25793870
results suggest that the primary effect of Omb is the blocking of Jak/STAT signaling by repressing transcription of upd which encodes the Jak receptor ligand Unpaired. PMID: 25781970
Since loss of omb disrupts horizontal system and vertical system cell development, we assume that HS/VS ontogeny is controlled by Omb target genes. PMID: 24912380
Dpp, via omb, promoted proliferation in the lateral and repressed proliferation in the medial wing disc. Omb controlled the regional proliferation rate by oppositely regulating transcription of the microRNA gene bantam in medial versus lateral wing disc. PMID: 23821035
four new omb mutations were isolated and characterized. PMID: 20033428
dysfunction of omb elicits hyperactivation of Hh signaling that causes an ectopic folding and local overgrowth in the wing columnar epithelium PMID: 17573067
OMB exprsssion in glial cells is required for correct axonal projection across the Drosophila inner optic chiasma. PMID: 18234176

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Subcellular Location

Nucleus.

Tissue Specificity

In third-instar larvae, expressed in the brain region that will develop into optic lobes and more weakly in the thoracic part of the ventral ganglion.

Database Links

KEGG: dme:Dmel_CG3578

STRING: 7227.FBpp0303589

UniGene: Dm.1683

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