Recombinant Drosophila melanogaster PDZ and LIM domain protein Zasp (Zasp52), partial

Code CSB-YP382939DLU
MSDS
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Source Yeast
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Code CSB-EP382939DLU
MSDS
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Source E.coli
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Code CSB-EP382939DLU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP382939DLU
MSDS
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Source Baculovirus
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Code CSB-MP382939DLU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Zasp52
Uniprot No.
Alternative Names
Zasp52; Zasp; CG30084; PDZ and LIM domain protein Zasp; Z band alternatively spliced PDZ-motif protein
Species
Drosophila melanogaster (Fruit fly)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Regulator of cell matrix adhesion having two related functions, one upstream of Actn organizing the Z line and the other downstream of integrins regulating assembly of integrin adhesion sites. Also required for the formation of myotendinous junctions in muscles.
Gene References into Functions
  1. analysis of Zasp52 nonmuscle expression and epithelial apicobasal localization PMID: 24846727
  2. Zasp is the first protein shown to co-activate alpha5beta1 integrins with talin and appears to do so in a manner distinct from known alphaIIbbeta3 integrin co-activators. PMID: 22992465
  3. Zasp52 and Zasp66 directly bind to alpha-actinin, and they can also form a ternary complex. PMID: 23505387
  4. Zasp52 is required for the association of alpha-actinin with the flight muscle Z-disc, and for normal sarcomere structure. PMID: 23271733
  5. stage- and tissue-specific expression in different muscle types for Zasp52 isoforms encoding different LIM domains. PMID: 21867777
  6. Our observations point to an important function for Zasp in the assembly of integrin adhesion sites both in cell culture and in tissues. PMID: 18166658
  7. Transcriptional analysis of dzasp has revealed six additional exons, with respect to the known 16, and multiple splice variants PMID: 19603185

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Subcellular Location
Cytoplasm, cytoskeleton.
Tissue Specificity
Expression is first detected in the proctodeum and the midgut primordium. In stage 11 embryos, expression is predominant in the leading edge of epidermal cells adjacent to the amnioserosa. Stage 12 embryos exhibit expression in the midgut and the leading
Database Links
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