Recombinant Drosophila melanogaster Polycomb protein PHO (pho), partial

Code CSB-YP848344DLU
MSDS
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Source Yeast
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Code CSB-EP848344DLU
MSDS
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Source E.coli
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Code CSB-EP848344DLU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP848344DLU
MSDS
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Source Baculovirus
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Code CSB-MP848344DLU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
pho
Uniprot No.
Alternative Names
pho; CG17743; Polycomb protein PHO; Protein pleiohomeotic; Transcription factor YY1 homolog
Species
Drosophila melanogaster (Fruit fly)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Polycomb group (PcG) protein that binds to the 5'-CNGCCATNNNNG-3' sequence found in the regulatory regions of many genes. PcG proteins act by forming multiprotein complexes, which are required to maintain the transcriptionally repressive state of homeotic genes throughout development. PcG proteins are not required to initiate repression, but to maintain it during later stages of development. They probably act via the methylation of histones, rendering chromatin heritably changed in its expressibility. Probably targets the Esc/E(z) complex to DNA. Necessary but not sufficient to recruit a functional PcG repressive complex that represses target genes, suggesting that the recruitment of the distinct PRC1 complex is also required to allow a subsequent repression.; Proposed core component of the chromatin remodeling Ino80 complex which is involved in transcriptional regulation, DNA replication and probably DNA repair.
Gene References into Functions
  1. Mutation in the protein-protein interaction domain (REPO domain) of Pho interferes with the dynamics of its interaction with Spt5. The transcriptional kinetics of the heat shock response is negatively affected by a mutation in the REPO domain of Pho PMID: 29208012
  2. Spt5 interacts both physically and genetically with the Polycomb Group (PcG) protein Pleiohomeotic (Pho) PMID: 23894613
  3. The TF homolog pair Pho/Yy1 seems to play very different roles in PcG targeting between Drosophila and mouse. PMID: 20353814
  4. Pleiohomeotic protein has a role in polycomb response element-mediated silencing for transcriptional repression PMID: 12370294
  5. This protein functions as a PcG protein in vivo. PMID: 12628927
  6. The mammalian transcription factor YY1 can function as a PcG protein in Drosophila resulting in transcriptional repression and correction of phenotypic defects caused by mutation of its homologue, Pleiohomeotic (PHO). PMID: 16052488
  7. Pleiohomeotic (PHO) and a multiprotein Polycomb core complex (PCC) bind highly cooperatively to polycomb response elements PMID: 16077005
  8. grainyhead interacts genetically with pleiohomeotic in a transgene-based, pairing-dependent silencing assay PMID: 16449654
  9. YY1 sequences 201-226 are necessary and sufficient for Polycomb group transcriptional repression in vivo. PMID: 17158804
  10. These results suggest that pho functions in maintaining expression of genes involved in the formation of ventral appendages and the proximo-distal axis. PMID: 18495104
  11. Pho is a general component of the maintenance machinery, since the majority of Pc targets are also associated with Pho (Pleiohomeotic )binding. PMID: 18773083
  12. a DNA-binding complex requiring Pho is differentially regulated to facilitate epigenetic transcriptional memory of both the active and the repressed state PMID: 19029043

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Subcellular Location
Nucleus.
Database Links

KEGG: dme:Dmel_CG17743

STRING: 7227.FBpp0088268

UniGene: Dm.1406

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