pho Antibody

^{Rare Species}

Datasheet

Code	CSB-PA848344XA01DLU
Size	US$299
Image	Western Blot Positive WB detected in Recombinant protein All lanes: pho antibody at 1:2000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 45 kDa Observed band size: 45 kDa
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Target Background

Product Details

Uniprot No.

Q8ST83

Target Names

pho

Alternative Names

Polycomb protein PHO (Protein pleiohomeotic) (Transcription factor YY1 homolog) pho CG17743

Species Reactivity

Drosophila melanogaster (Fruit fly)

Immunogen

Recombinant Drosophila melanogaster (Fruit fly) pho protein (1-250aa )

Immunogen Species

Drosophila melanogaster (Fruit fly)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Protein G

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB

Protocols

ELISA Protocol
Western Blotting(WB) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

Polycomb group (PcG) protein that binds to the 5'-CNGCCATNNNNG-3' sequence found in the regulatory regions of many genes. PcG proteins act by forming multiprotein complexes, which are required to maintain the transcriptionally repressive state of homeotic genes throughout development. PcG proteins are not required to initiate repression, but to maintain it during later stages of development. They probably act via the methylation of histones, rendering chromatin heritably changed in its expressibility. Probably targets the Esc/E(z) complex to DNA. Necessary but not sufficient to recruit a functional PcG repressive complex that represses target genes, suggesting that the recruitment of the distinct PRC1 complex is also required to allow a subsequent repression.; Proposed core component of the chromatin remodeling Ino80 complex which is involved in transcriptional regulation, DNA replication and probably DNA repair.

Gene References into Functions

Mutation in the protein-protein interaction domain (REPO domain) of Pho interferes with the dynamics of its interaction with Spt5. The transcriptional kinetics of the heat shock response is negatively affected by a mutation in the REPO domain of Pho PMID: 29208012
Spt5 interacts both physically and genetically with the Polycomb Group (PcG) protein Pleiohomeotic (Pho) PMID: 23894613
The TF homolog pair Pho/Yy1 seems to play very different roles in PcG targeting between Drosophila and mouse. PMID: 20353814
Pleiohomeotic protein has a role in polycomb response element-mediated silencing for transcriptional repression PMID: 12370294
This protein functions as a PcG protein in vivo. PMID: 12628927
The mammalian transcription factor YY1 can function as a PcG protein in Drosophila resulting in transcriptional repression and correction of phenotypic defects caused by mutation of its homologue, Pleiohomeotic (PHO). PMID: 16052488
Pleiohomeotic (PHO) and a multiprotein Polycomb core complex (PCC) bind highly cooperatively to polycomb response elements PMID: 16077005
grainyhead interacts genetically with pleiohomeotic in a transgene-based, pairing-dependent silencing assay PMID: 16449654
YY1 sequences 201-226 are necessary and sufficient for Polycomb group transcriptional repression in vivo. PMID: 17158804
These results suggest that pho functions in maintaining expression of genes involved in the formation of ventral appendages and the proximo-distal axis. PMID: 18495104
Pho is a general component of the maintenance machinery, since the majority of Pc targets are also associated with Pho (Pleiohomeotic )binding. PMID: 18773083
a DNA-binding complex requiring Pho is differentially regulated to facilitate epigenetic transcriptional memory of both the active and the repressed state PMID: 19029043

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Subcellular Location

Nucleus.

Database Links

KEGG: dme:Dmel_CG17743

STRING: 7227.FBpp0088268

UniGene: Dm.1406

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