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Recombinant Drosophila melanogaster Polycomb protein Scm (Scm), partial

Recombinant Drosophila melanogaster Polycomb protein Scm (Scm), partial

Code	CSB-YP895612DLU
MSDS	MSDS Datasheet
Size	Pls inquire
Source	Yeast
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Code	CSB-EP895612DLU
MSDS	MSDS Datasheet
Size	Pls inquire
Source	E.coli
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Code	CSB-EP895612DLU-B
MSDS	MSDS Datasheet
Size	Pls inquire
Source	E.coli
Conjugate	Avi-tag Biotinylated E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code	CSB-BP895612DLU
MSDS	MSDS Datasheet
Size	Pls inquire
Source	Baculovirus
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Code	CSB-MP895612DLU
MSDS	MSDS Datasheet
Size	Pls inquire
Source	Mammalian cell
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Product Details
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Customer Reviews and Q&A
Target Background

Product Details

Purity

>85% (SDS-PAGE)

Target Names

Scm

Uniprot No.

Q9VHA0

Alternative Names

Scm; CG9495; Polycomb protein Scm; Sex comb on midleg protein

Species

Drosophila melanogaster (Fruit fly)

Protein Length

Partial

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function

Polycomb group (PcG) protein. PcG proteins act by forming multiprotein complexes, which are required to maintain the transcriptionally repressive state of homeotic genes throughout development. PcG proteins are not required to initiate repression, but to maintain it during later stages of development. They probably act via the methylation of histones, rendering chromatin heritably changed in its expressibility.

Gene References into Functions

loss-of-function mutations in Scm result in non-autonomous tissue overgrowth in Drosophila, and determine that Scm is essential for ommatidium development and important for cell survival in Drosophila PMID: 25772304
Scm coordinates PcG complexes and polymerizes to produce broad domains of PcG silencing PMID: 26063573
SUMO negatively regulates Scm function by impeding its recruitment to the Ubx major PRE. PMID: 21278366
Data show that Sex Comb on Midleg (SCM) associates with a Polycomb response element upstream of the Ubx gene which also binds PRC1, PRC2, and the DNA-binding PcG protein Pleiohomeotic. PMID: 20351181
SCM interacts and functions with Polycomb repressive complex 1 in gene silencing. PMID: 15280237
polymeric Scm complexes could extend beyond the local domains of polymeric Ph complexes on chromatin, possibly playing a role in long range repression PMID: 15905166
Data show that the MBT domain of Sex comb on midleg and its methyl-lysine-binding activity are required for repression of Hox genes. PMID: 17932512
Testing the role of this structure in male mating success in Drosophila melanogaster, the sex comb was removed by expressing the female-specific isoform of the sex determination gene transformer in the tarsal segments of male legs. PMID: 18213513