Recombinant Drosophila melanogaster Probable insulin-like peptide 2 (Ilp2)

Code CSB-YP894733DLU
MSDS
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Source Yeast
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Code CSB-EP894733DLU
MSDS
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Source E.coli
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Code CSB-EP894733DLU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP894733DLU
MSDS
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Source Baculovirus
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Code CSB-MP894733DLU
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Ilp2
Uniprot No.
Alternative Names
Ilp2; IRP; CG8167; Probable insulin-like peptide 2; dILP2; Insulin-related peptide 2) [Cleaved into: Probable insulin-like peptide 2 A chain; Probable insulin-like peptide 2 B chain]
Species
Drosophila melanogaster (Fruit fly)
Expression Region
27-137
Target Protein Sequence
TLCS EKLNEVLSMV CEEYNPVIPH KRAMPGADSD LDALNPLQFV QEFEEEDNSI SEPLRSALFP GSYLGGVLNS LAEVRRRTRQ RQGIVERCCK KSCDMKALRE YCSVVRN
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Plays a role in regulating body size by increasing cell size and cell number of individual organs. Probably mediates its growth effects by acting as a ligand for the insulin receptor and transducing a signal via the Chico/PI3K/Akt(PKB) pathway.
Gene References into Functions
  1. Immunosenescence in Drosophila suggests its possible mechanisms that involve changes in insulin/IGF-1 signaling. (Review) PMID: 30134574
  2. Using two long-lived IIS models, namely Drosophila lacking three insulin-like peptides (dilp2-3,5(-/-)) and mice lacking insulin receptor substrate 1 (Irs1(-/-)), and two independent translation assays, polysome profiling and radiolabeled amino acid incorporation, we show that reduced IIS lowers translation in these organisms PMID: 27452396
  3. dPKD mediated secretion of dILP2 from neurosecretory cells during development is necessary for normal larval growth. PMID: 29247620
  4. Results found that virgin females with knockout mutations of Ilp2, Ilp3 and Ilp5 show high mating success rate with higher locomotion. PMID: 26617266
  5. Knockdown of the Drosophila orthologues of human T2DM risk genes, including GLIS3 and BCL11A, revealed roles of these Drosophila genes in Ilp2 production or secretion. PMID: 25101872
  6. Under hypoxic conditions, Sima nuclear localization in the fat body is associated with lipid droplet aggregation and impaired lipid mobilization to oenocytes. The fat body in turn, perhaps through a secreted signal, inhibits Dilp2 secretion from the larval brain, associated with reduced insulin receptor signaling in peripheral tissues. PMID: 25541690
  7. These results indicate that adiponectin receptor in insulin producing cells regulates insulin secretion and controls glucose and lipid metabolism in Drosophila melanogaster. PMID: 23874700
  8. dMyc in fat body affects the release of DILP2 from the insulin producing cells. PMID: 23608455
  9. show that the DILP-producing median neurosecretory cells are required to mediate nearly all of the response of lifespan and some of the response of fecundity to yeast levels PMID: 20156206
  10. brain insulin-producing cells are the main systemic supply of insulin during larval growth PMID: 12004130
  11. despite the correlation between reduced dilp2 mRNA levels and lifespan-extension often observed, DILP2 reduction is not sufficient to extend lifespan PMID: 19005568

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Subcellular Location
Secreted.
Protein Families
Insulin family
Tissue Specificity
Broadly expressed at a low level in the embryonic mesoderm, beginning at stage 12. Expressed at a high level in the embryonic anterior midgut, with expression diminishing at late stage 16. Expressed at a low level in larval imaginal disks. Expressed at a
Database Links

KEGG: dme:Dmel_CG8167

STRING: 7227.FBpp0076058

UniGene: Dm.2285

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