Recombinant Drosophila melanogaster Protein 60A (gbb)

Code CSB-YP338804DLU
MSDS
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Source Yeast
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Code CSB-EP338804DLU
MSDS
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Source E.coli
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Code CSB-EP338804DLU-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP338804DLU
MSDS
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Source Baculovirus
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Code CSB-MP338804DLU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
gbb
Uniprot No.
Alternative Names
gbb; 60A; gbb-60A; TGFb-60A; CG5562Protein 60A; Protein glass bottom boat
Species
Drosophila melanogaster (Fruit fly)
Expression Region
336-455
Target Protein Sequence
SVSPN NVPLLEPMES TRSCQMQTLY IDFKDLGWHD WIIAPEGYGA FYCSGECNFP LNAHMNATNH AIVQTLVHLL EPKKVPKPCC APTRLGALPV LYHLNDENVN LKKYRNMIVK SCGCH
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Required for the growth of imaginal tissues and for patterning of the adult wing.
Gene References into Functions
  1. The results indicate that high-fat diet-induced Gbb signaling provokes insulin resistance by increasing tribbles expression. PMID: 27484164
  2. propose that Gbb/Cmpy corelease from presynaptic terminals defines a neuronal protransmission signal PMID: 25453556
  3. Glial Mav modulates muscle expression of Gbb. PMID: 22959350
  4. Studies indicate that glass bottom boat (Gbb) can be processed singly at a prodomain site or a main site to generate two active isoforms, Gbb15 and Gbb38. PMID: 22472647
  5. Data indicate that Gbb38, the large ligand form of the glass bottom boat (Gbb) exhibited greater signaling activity and a longer range than the shorter form Gbb15. PMID: 22472650
  6. Data demonstrate that crimpy (cmpy; CG13253) limits neuronal Gbb activity to permit appropriate regulation of neuromuscular junction growth. PMID: 21750037
  7. The dRich coordinates the Gbb-dependent modulation of synaptic growth and function with postsynaptic development. PMID: 21041451
  8. Gbb/BMP signaling is a new pathway critical for positive regulation of nutrient storage and energy homeostasis during development. PMID: 19914231
  9. The BMP homolog Gbb provides a retrograde signal that regulates synaptic growth at the Drosophila neuromuscular junction. PMID: 12873382
  10. Gbb globally regulates NMJ function by controlling both the growth and transmitter release properties of the synapse. PMID: 14507784
  11. This present study demonstrates that the central synapses that coordinate motoneuron activation are also reliant on Gbb-dependent retrograde signaling. PMID: 15295025

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Subcellular Location
Secreted.
Protein Families
TGF-beta family
Tissue Specificity
Expressed in cells of the developing foregut and hindgut during germ band retraction and later embryonic stages. Expressed in the wing disk, mainly in the posterior compartment in the pteropleural and medial regions extending into the progenitors of the s
Database Links

KEGG: dme:Dmel_CG5562

STRING: 7227.FBpp0072036

UniGene: Dm.4758

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