Recombinant Drosophila melanogaster Protein Gawky (gw), partial

Code CSB-YP837395DLU
MSDS
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Source Yeast
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Code CSB-EP837395DLU
MSDS
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Source E.coli
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Code CSB-EP837395DLU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP837395DLU
MSDS
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Source Baculovirus
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Code CSB-MP837395DLU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
gw
Uniprot No.
Alternative Names
gw; GW182; CG31992Protein Gawky
Species
Drosophila melanogaster (Fruit fly)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Required for gene silencing mediated by micro-RNAs (miRNAs). Silences both polyadenylated and deadenylated mRNAs. Required for miRNA-mediated translational repression and mRNA decay. Not required for miRNA target recognition. Necessary to initiate but not to maintain silencing. Promotes mRNA deadenylation through the recruitment of the CCR4-NOT and PAN complexes and promotes decapping by the DCP1-DCP2 complex. Dissociates from silenced mRNAs after deadenylation. Required for completion of nuclear divisions during early embryonic development.
Gene References into Functions
  1. This study demonistryed that GW182's gene silencing activity functions as a rheostat for PDFR signaling and thus profoundly impacts the circadian neural network and its response to environmental inputs. PMID: 23583112
  2. miRNA-mediated translational repression and degradation are mechanistically linked and are triggered through the interactions of GW182 with poly(A)-binding protein and deadenylases. PMID: 23172285
  3. Gawky plays a critical role in miRNA-mediated gene silencing. (Review) PMID: 23224968
  4. MicroRNAs utilize at least three distinct silencing pathways: GW182-mediated deadenylation and GW182-dependent and -independent repression of early translation initiation. PMID: 23123195
  5. Data demonstrate that in human and Drosophila melanogaster cells, the critical repressive features of both the N-terminal and C-terminal effector domains of GW182 proteins are Gly/Ser/Thr-Trp (G/S/TW) or Trp-Gly/Ser/Thr (WG/S/T) motifs. PMID: 21984184
  6. Multiple GW/WG repeats present in the minimal N-terminal repressor domain of Drosophila GW182 are required for target mRNA repression. PMID: 20530530
  7. GW182 links the miRNA pathway to mRNA degradation PMID: 16815998
  8. This suggests that gawky represents a previously unknown member of a small group of genes that need to be expressed zygotically during early embryo development. PMID: 16880270
  9. deletion of the RNA recognition motif impairs the silencing activity of GW182 in a miRNA target-specific manner, indicating that this domain contributes to silencing. PMID: 19295135
  10. Using both tethered function and complementation assays, study identified 3 independent domains of the Drosophila GW182 protein (also termed Gawky) that are sufficient to repress mRNA. PMID: 19304924

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Subcellular Location
Cytoplasm, P-body.
Protein Families
GW182 family
Database Links

KEGG: dme:Dmel_CG31992

STRING: 7227.FBpp0088165

UniGene: Dm.1403

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