Recombinant Drosophila melanogaster Protein TIS11 (Tis11)

Code CSB-YP344105DLU
MSDS
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Source Yeast
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Code CSB-EP344105DLU
MSDS
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Source E.coli
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Code CSB-EP344105DLU-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP344105DLU
MSDS
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Source Baculovirus
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Code CSB-MP344105DLU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Tis11
Uniprot No.
Alternative Names
Tis11; TIScc1; CG4070; Protein TIS11; dTIS11
Species
Drosophila melanogaster (Fruit fly)
Expression Region
1-436
Target Protein Sequence
MSADILQKSR EQDDSHYFER GDISKYVTMN DHLGDFDCNE VRKEIRMLLA HGANLDQQHQ QQPHRHHGGL TRTISQPAQL IQQQQQQHQQ QQQQQPAVAS LVTITENLGN MNLHRKLERT QSEPLPPQQP MNTSRYKTEL CRPFEEAGEC KYGEKCQFAH GSHELRNVHR HPKYKTEYCR TFHSVGFCPY GPRCHFVHNA DEARAQQAAQ AAKSSTQSQS QSQQSSSQNF SPKSNQSSNQ SSNSSSSSSS SGGGGGGGNS INNNNGSQFY LPLSPPLSMS TGSDRESPTG SLSLSPTNSL TSFPFHDALQ HGYLASNGAK SNSSASSTSS ASGMGLGMSM GIGQGMIIGQ GLGMGHHGPA TPPESPNVPI SPVHTPPPYD VVVSGSGAGN NSVGSKQLLQ KSVSTPMQQE DTPRLPVFNR LSSGVEAYQQ QSNLGL
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Gene References into Functions
  1. Data indicate that Tis11 protein can affect post-transcriptional gene expression in adult flies by regulating mRNA decay. PMID: 25342740
  2. the Drosophila melanogaster dTIS11 protein is short-lived due to its rapid ubiquitin-independent degradation by the proteasome. PMID: 25246635
  3. Nuclear export of TIS11 proteins is mediated by CRM1 through diverging nuclear export signals, while their nuclear import mechanism may rely on a highly conserved signal whose activity is negatively regulated by ZnF2 folding. PMID: 23951221
  4. overexpression of dTIS11 does not promote the localization of ARE-containing mRNAs in P-bodies but rather decreases the accumulation of CecA1 mRNA in these structures by enhancing the degradation process PMID: 22932903
  5. study identified a novel mRNA target of DTIS11, eya, and provided evidence that DTIS11 regulates eye development by interacting with and destabilizing eya mRNA, especially during embryogenesis PMID: 22553461
  6. The functionality of CecA1 ARE relies on its ability to recruit TIS11 protein, which accelerates CecA1 mRNA deadenylation and decay PMID: 19176529

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Subcellular Location
Nucleus.
Database Links

KEGG: dme:Dmel_CG4070

STRING: 7227.FBpp0073516

UniGene: Dm.4329

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