Recombinant Drosophila melanogaster Protein arginine N-methyltransferase 5 (csul), partial

Code CSB-YP866187DLU
MSDS
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Source Yeast
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Code CSB-EP866187DLU
MSDS
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Source E.coli
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Code CSB-EP866187DLU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP866187DLU
MSDS
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Source Baculovirus
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Code CSB-MP866187DLU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
csul
Uniprot No.
Alternative Names
csul; DART5; CG3730Protein arginine N-methyltransferase 5; EC 2.1.1.-; JBP1 homolog; Protein arginine N-methyltransferase capsuleen
Species
Drosophila melanogaster (Fruit fly)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Arginine methyltransferase that can both catalyze the formation of omega-N monomethylarginine (MMA) and symmetrical dimethylarginine (sDMA). Specifically mediates the symmetrical dimethylation of arginine residues in the small nuclear ribonucleoproteins SmD1 and SmD3. Required for arginine symmetrical dimethylation of piwi family proteins, piwi, aub and AGO3, during germline development. Required during oogenesis for pole cell formation in the pathway controlled by oskar (osk) and for abdominal segments during early embryogenesis. Involved in nanos (nos) and germ cell mRNAs localization.
Gene References into Functions
  1. Studies indicate that protein arginine methyltransferase 5 (PRMT5) plays a role in circadian clocks regulated alternative splicing process. PMID: 21941124
  2. results demonstrate a key role for PRMT5 in the regulation of alternative splicing PMID: 20962777
  3. in germline cells, piRNAs are quality-controlled by dPRMT5 that modifies PIWI proteins, in tight association with Tud PMID: 19959991
  4. Dart5 has a role in germ-cell development. PMID: 16753561
  5. Capsuleen is essential for methylation of spliceosomal Sm proteins and germ cell formation. PMID: 17164419

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Subcellular Location
Cytoplasm.
Protein Families
Class I-like SAM-binding methyltransferase superfamily, Protein arginine N-methyltransferase family
Tissue Specificity
Expressed only in ovaries.
Database Links

KEGG: dme:Dmel_CG3730

STRING: 7227.FBpp0088810

UniGene: Dm.3058

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