Recombinant Drosophila melanogaster Protein bicaudal D (BicD), partial

Code CSB-YP321454DLU
MSDS
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Source Yeast
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Code CSB-EP321454DLU
MSDS
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Source E.coli
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Code CSB-EP321454DLU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP321454DLU
MSDS
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Source Baculovirus
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Code CSB-MP321454DLU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
BicD
Uniprot No.
Alternative Names
BicD; CG6605Protein bicaudal D
Species
Drosophila melanogaster (Fruit fly)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
This protein is essential for differentiation. It may play a role in localizing of Nanos (a maternal determinant) activity in oocytes. During oogenesis, plays a specific role, together with Rab6 but independently of Sec5, in the polarization of the oocyte microtubule cytoskeleton, in oskar mRNA localization and in the anterodorsal secretion of grk. Plays a role in the biogenesis of annulate lamellae containing nuclear pore complex components.
Gene References into Functions
  1. 2.2 A resolution crystal structure of a cargo-binding region of the dynein adaptor Bicaudal-D (BicD), which reveals a parallel coiled-coil homodimer PMID: 23723415
  2. Results implicate BicD as a new player in clathrin-associated trafficking processes and show a novel requirement for microtubule-based motor transport in the synaptic vesicle cycle. PMID: 20111007
  3. component of a selective dynein motor complex that drives transcript localization in a variety of tissues PMID: 11740552
  4. Various processes in which BicD is involved during embryogenesis (review) PMID: 16320833
  5. while BicD might contribute to recruitment of dynein to the cargo it is not absolutely required for such dynein localization PMID: 19018277

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Subcellular Location
Cytoplasm, cytoskeleton.
Protein Families
BicD family
Tissue Specificity
In ovaries, expressed in oocyte and nurse cells.
Database Links

KEGG: dme:Dmel_CG6605

STRING: 7227.FBpp0303259

UniGene: Dm.3232

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