Recombinant Drosophila melanogaster Protein bowel (bowl), partial

Code CSB-YP895179DLU
MSDS
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Source Yeast
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Code CSB-EP895179DLU
MSDS
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Source E.coli
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Code CSB-EP895179DLU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP895179DLU
MSDS
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Source Baculovirus
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Code CSB-MP895179DLU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
bowl
Uniprot No.
Alternative Names
bowl; CG10021; Protein bowel; Brother of odd with entrails limited
Species
Drosophila melanogaster (Fruit fly)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Putative transcription factor. Required for leg joint formation, acting downstream of Notch to pattern the leg tarsal segments. Functions in the terminal pathway during embryogenesis, acting downstream of tll in the posterior of the embryo. Acts in a hierarchy downstream of drm and lin during foregut and hindgut patterning and morphogenesis. Involved in cell rearrangement during elongation of the embryonic hindgut. Regulates expression of hindgut patterning genes to establish the small intestine region of the embryonic hindgut. Required in the foregut for spatially localized gene expression and morphogenesis of the proventriculus.
Gene References into Functions
  1. we find that C15 interacts physically with the Dll activator through contacts between their homeodomain and binds competitively with Dll to adjacent cognate sites on LAE, adding potential new layers of regulation by C15. Lastly, we show that C15 and Bowl activities regulate also rn expression PMID: 28394894
  2. Lines acts antagonistically to bowl in hub specification, establishing bowl as a positively acting factor in the development of the testis niche. PMID: 21486923
  3. Bowl is part of a genetic program that suppresses the formation of supernumerary appendages specifically in the fly's head. PMID: 18662773
  4. Reciprocal roles are reported for bowl and lin in specifying the peripodial epithelium and the disc proper of the wing. PMID: 18701548
Subcellular Location
Nucleus.
Tissue Specificity
Expressed at the termini of the blastoderm embryo in three domains; strongly expressed at the posterior pole, relatively weakly expressed at the anterior pole and expressed in a broad transverse stripe just anterior to the presumptive cephalic furrow. Sub
Database Links

KEGG: dme:Dmel_CG10021

STRING: 7227.FBpp0297869

UniGene: Dm.4025

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