Recombinant Drosophila melanogaster Protein bunched, class 1/class 3/D/E isoforms (bun)

Code CSB-YP634511DLU
MSDS
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Source Yeast
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Code CSB-EP634511DLU
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Source E.coli
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Code CSB-EP634511DLU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP634511DLU
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Source Baculovirus
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Code CSB-MP634511DLU
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
bun
Uniprot No.
Alternative Names
bun; shs; CG42281Protein bunched; class 1/class 3/D/E isoforms; Protein shortsighted
Species
Drosophila melanogaster (Fruit fly)
Expression Region
1-219
Target Protein Sequence
MKTETGSNNN NTTVVNMDFD MYPSISGKQQ DPVREVVMKY IDYFLPDASG TSAVAIDNKI EQAMDLVKSH LMIAVREEVE VLKERISELM DKINKLELEN SILKSNIPQE TLQQLQLQLQ LAAPPATPAI QAAPAVQSVV APAAAGQAVQ QQAAGAVAVT GVATSPASAV VPTSIPNGSA ENGSSAVESA AVSVEQQVQQ VTSAAAAAAS VVTANGPMS
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Probable transcription factor required for peripheral nervous system morphogenesis, eye development and oogenesis. May be required for the transmission of the dpp signal and for a morphogenetic movement of the medulla in the brain that reorients the second optic lobe relative to the first. Plays a role in determining proper dorsal cell fates leading to the formation of the dorsal appendages.
Gene References into Functions
  1. The data suggest the model that Bunched and Madm functionally interact with the TOR pathway in the cytoplasm to regulate the growth and subsequent division of intestinal stem cells. PMID: 26323255
  2. Bunched, a negative regulator of Notch, is involved in sleep homeostasis through a neuron-glia signal pathway. PMID: 21549599
  3. bun regulates the level of Notch stimulation to position the boundary between centripetally migrating and stationary columnar follicle cells PMID: 16223477
  4. In anterior follicle cells exposed to a Dpp morphogen gradient, high and low levels of slbo and bun, respectively, are established by modulation of Notch signaling to direct threshold cell fates. PMID: 17383627
  5. BunDN has dominant negative activity useful to identify bun functions and genetic interactions that occur during tissue patterning. PMID: 17600691
  6. we hypothesize that the long TSC-22 protein is a functional homolog of BunA in growth regulation, and that it is antagonized by the short TSC-22 protein PMID: 18226226
  7. Drosophila TSC-22/DIP/Bun proteins are necessary for cellular growth, proliferation, and survival both in culture and in an epithelial context. PMID: 18375761
  8. bun acts as a specific factor in regulating neuroblast mitotic activity during the development of alpha/beta neurons. PMID: 19328837

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Subcellular Location
Cytoplasm. Nucleus.
Protein Families
TSC-22/Dip/Bun family
Tissue Specificity
Anterior dorsal follicle cells at stages 10-12 of oogenesis.
Database Links

UniGene: Dm.7349

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