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Recombinant Drosophila melanogaster Protein charlatan (chn), partial

Recombinant Drosophila melanogaster Protein charlatan (chn), partial

Code	CSB-YP773570DLU
MSDS	MSDS Datasheet
Size	Pls inquire
Source	Yeast
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Code	CSB-EP773570DLU
MSDS	MSDS Datasheet
Size	Pls inquire
Source	E.coli
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Code	CSB-EP773570DLU-B
MSDS	MSDS Datasheet
Size	Pls inquire
Source	E.coli
Conjugate	Avi-tag Biotinylated E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code	CSB-BP773570DLU
MSDS	MSDS Datasheet
Size	Pls inquire
Source	Baculovirus
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Code	CSB-MP773570DLU
MSDS	MSDS Datasheet
Size	Pls inquire
Source	Mammalian cell
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Product Details
Related Products
Customer Reviews and Q&A
Target Background

Product Details

Purity

>85% (SDS-PAGE)

Target Names

chn

Uniprot No.

Q7YU81

Alternative Names

chn; CG11798Protein charlatan

Species

Drosophila melanogaster (Fruit fly)

Protein Length

Partial

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function

Probable transcription factor involved in the development of the adult pattern of macrochaetae. Required for accumulation of achaete (ac) and scute (sc) in proneural clusters. Probably acts by binding to the proneural cluster-specific enhancers of the ac/sc complex and increasing enhancer efficiency, thereby acting as a stimulator of ac/sc expression in proneural clusters. Also required for correct development of the embryonic/larval peripheral nervous system (PNS).

Gene References into Functions

Gene expression profiling identifies the zinc-finger protein Charlatan as a regulator of intestinal stem cells in Drosophila. PMID: 24961799
Chn is a dual functional regulator of transcription. PMID: 21762412
mutation decreases the accumulation of Sc in proneural clusters, affecting notum macrochaetae, overexpression enhances ac/sc PMID: 15703278
under EGF signaling, double negative regulation mediated by the Ebi/SMRTER corepressor complex and an NRSF/REST-like factor, Chn, maintains inductive activity in developing photoreceptor cells by promoting Dl expression PMID: 16763555

Subcellular Location

Nucleus.

Tissue Specificity

Expressed in the PNS and CNS. In early blastoderm stages, it is ubiquitously expressed, then, before stage 5, it disappears from the poles of the embryo and faint stripes are visible. At stage 5, it also accumulates in the dorsal region, cephalic furrow e

Database Links

KEGG: dme:Dmel_CG11798

STRING: 7227.FBpp0293744

UniGene: Dm.11696

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