Recombinant Drosophila melanogaster Protein daughterless (da), partial

Code CSB-YP320868DLU
MSDS
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Source Yeast
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Code CSB-EP320868DLU
MSDS
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Source E.coli
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Code CSB-EP320868DLU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP320868DLU
MSDS
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Source Baculovirus
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Code CSB-MP320868DLU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
da
Uniprot No.
Alternative Names
da; CG5102Protein daughterless
Species
Drosophila melanogaster (Fruit fly)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Daughterless/Achaete-scute complex heterodimers act as transcriptional activators of neural cell fates and are involved in sex determination.
Gene References into Functions
  1. Daughterless and Tcf4 are expressed in postmitotic neurons in Drosophila melanogaster and mice, respectively, where they function to restrict neurite branching and synapse formation in part by restricting the expression of the cell adhesion molecule Neurexin. PMID: 27050508
  2. We explain this paradox by demonstrating that Da induces the differentiation factor Prospero (Pros) whose asymmetric segregation is essential for differentiation in one of the two daughter cells. PMID: 24804774
  3. we show that during the development of epithelial cells in the imaginal discs, the G2/M transition, and hence cell proliferation, is controlled by Emc via Da. In eukaryotic cells, the main activator of this transition is the Cdc25 phosphatase, string PMID: 24651265
  4. Da transactivation domains play essential roles in neurogenesis and in E(spl)-mediated repression. PMID: 22949507
  5. lilli contributes to ato auto-regulation in the morphogenetic furrow by first regulating the expression of da prior to ato. PMID: 22275119
  6. coordinates somatic cell proliferation, differentiation and germline cyst survival during follicle formation in Drosophila PMID: 12070099
  7. Da confers neuronal identity to epithelial cells in the wing margin. PMID: 16554363
  8. The expression of Dap was found to be controlled by the epidermal growth factor receptor signaling-regulated transcription factor Pointed (Pnt) and the proneural basic helix-loop-helix proteins Atonal (Ato) and Daughterless (Da). PMID: 17296729
  9. Daughterless dictates Twist activity in a context-dependent manner during somatic myogenesis. PMID: 18407256
  10. Study shows that the Drosophila class I factor Daughterless (Da) is upregulated by Hedgehog (Hh) and Decapentaplegic (Dpp) signalling during retinal neurogenesis. PMID: 18758436

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Subcellular Location
Nucleus.
Tissue Specificity
Ubiquitous.
Database Links

KEGG: dme:Dmel_CG5102

STRING: 7227.FBpp0304504

UniGene: Dm.388

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