Recombinant Drosophila melanogaster Protein escargot (esg)

Code CSB-YP326585DLU
MSDS
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Source Yeast
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Code CSB-EP326585DLU
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Source E.coli
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Code CSB-EP326585DLU-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP326585DLU
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Source Baculovirus
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Code CSB-MP326585DLU
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
esg
Uniprot No.
Alternative Names
esg; flg; CG3758Protein escargot; Protein fleabag
Species
Drosophila melanogaster (Fruit fly)
Expression Region
1-470
Target Protein Sequence
MHTVEDMLVE KNYSKCPLKK RPVNYQFEAP QNHSNTPNEP QDLCVKKMEI LEENPSEELI NVSDCCEDEG VDVDHTDDEH IEEEDEDVDV DVDSDPNQTQ AAALAAAAAV AAAAAASVVV PTPTYPKYPW NNFHMSPYTA EFYRTINQQG HQILPLRGDL IAPSSPSDSL GSLSPPPHHY LHGRASSVSP PMRSEIIHRP IGVRQHRFLP YPQMPGYPSL GGYTHTHHHH APISPAYSEN SYYSMRSMTP ESSCSSSLPE DLSLKHKNLN LNLNTSQPGE QAAAKTGDMS PETMPNASAK KDKNQPPRYQ CPDCQKSYST FSGLTKHQQF HCPAAEGNQV KKSFSCKDCD KTYVSLGALK MHIRTHTLPC KCNLCGKAFS RPWLLQGHIR THTGEKPFSC QHCHRAFADR SNLRAHLQTH SDIKKYSCTS CSKTFSRMSL LTKHSEGGCP GGSAGSSSSS ELNYAGYAEP
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Transcription factor that can both stimulate and repress transcription. Binds to the consensus DNA sequence 5'-A/GCAGGTG-3'. Regulates cell motility and adhesion during tracheal morphogenesis by stimulating transcription of the DE-cadherin gene shg at branch tips, thereby promoting tracheal tube fusion. Maintains diploidy in imaginal cells by inhibiting the transcription of genes required for endoreplication. Required for development of the genital disk and acts as an intrinsic determinant of wing cell fate. The somatic protein is required for maintenance of male germ cells. Acts with other members of the snail protein family to control embryonic central nervous system development.
Gene References into Functions
  1. Esg controls specification of cell types in trachea and suppresses ERK signaling in fusion cells to control cells' differential migratory behavior. PMID: 27742749
  2. Escargot and Scratch regulate neural commitment by antagonizing Notch activity in Drosophila sensory organs PMID: 27471258
  3. CySCs mutant for esg are not maintained as stem cells, but remain capable of differentiating normally along the cyst cell lineage. In contrast, esg mutant CySCs that give rise to a closely related lineage, the apical hub cells, cannot maintain hub cell identity. this dual regulatory role is due to simultaneous control by Esg of overlapping genetic programs. Similar results were obtained with intestinal stem cells. PMID: 27077690
  4. Robust intestinal homeostasis relies on cellular plasticity in enteroblasts mediated by miR-8-Escargot switch. PMID: 26077448
  5. Cell type-specific transcriptome analysis combined with Dam-ID binding studies identified Esg as a major repressor of differentiation genes in stem and progenitor cells. PMID: 25298397
  6. Authors demonstrate that Esg plays a pivotal role in intestinal homeostasis, maintaining the stem cell pool while influencing fate decisions through modulation of Notch activity. PMID: 25433031
  7. Escargot protein as an essential factor is required in hub cells for maintaining niche integrity by blocking conversion of hub cells to the cyst cell lineage. PMID: 24794442
  8. Escargot directly interact with Daughtherless and its mammalian homologue HEB and eliminate them through siah-1 mediated protein degradation. PMID: 20647756
  9. role as an enhancer trap that monitors male-specific gene expression in germ cells PMID: 11902678
  10. snail is required for Drosophila gastrulation and is not replaceable by Escargot or Worniu. PMID: 15110709
  11. esg must be ectopically expressed during nervous system development to reduce seizure susceptibility in adult drosophila. PMID: 15654097

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Subcellular Location
Nucleus.
Protein Families
Snail C2H2-type zinc-finger protein family
Tissue Specificity
Expression is complex and dynamic. In early embryogenesis, expression begins on the dorsal side of the embryo. Expressed in a pattern of longitudinal stripes early in germband elongation. Later in embryogenesis, expression is in cells that correspond to t
Database Links

KEGG: dme:Dmel_CG3758

STRING: 7227.FBpp0080293

UniGene: Dm.4764

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