Recombinant Drosophila melanogaster Protein hairy (h)

Code CSB-YP318457DLU
MSDS
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Source Yeast
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Code CSB-EP318457DLU
MSDS
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Source E.coli
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Code CSB-EP318457DLU-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP318457DLU
MSDS
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Source Baculovirus
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Code CSB-MP318457DLU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
h
Uniprot No.
Alternative Names
h; CG6494Protein hairy
Species
Drosophila melanogaster (Fruit fly)
Expression Region
1-337
Target Protein Sequence
MVTGVTAANM TNVLGTAVVP AQLKETPLKS DRRSNKPIME KRRRARINNC LNELKTLILD ATKKDPARHS KLEKADILEK TVKHLQELQR QQAAMQQAAD PKIVNKFKAG FADCVNEVSR FPGIEPAQRR RLLQHLSNCI NGVKTELHQQ QRQQQQQSIH AQMLPSPPSS PEQDSQQGAA APYLFGIQQT ASGYFLPNGM QVIPTKLPNG SIALVLPQSL PQQQQQQLLQ HQQQQQQLAV AAAAAAAAAA QQQPMLVSMP QRTASTGSAS SHSSAGYESA PGSSSSCSYA PPSPANSSYE PMDIKPSVIQ RVPMEQQPLS LVIKKQIKEE EQPWRPW
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Pair-rule protein that regulates embryonic segmentation and adult bristle patterning. Transcriptional repressor of genes that require a bHLH protein for their transcription (e.g. ftz).
Gene References into Functions
  1. Hairy induces widespread histone deacetylation and inhibits the recruitment of basal machinery without inducing chromatin compaction. PMID: 21353562
  2. In this study, the authors show that Dgrn is a negative regulator of the repressor Hairy and its corepressor Groucho (Gro/transducin-like enhancer (TLE)) during embryonic segmentation and neurogenesis. PMID: 21343912
  3. Studies demonstrate a novel role for Hairy in restriction of the terminal cell fate by limiting the domain of bnl expression in surrounding muscle cells such that only a single dorsal branch cell becomes specified as a terminal cell. PMID: 21152432
  4. identification as a quantitative trait locus for drosophila sensory bristle number PMID: 12242230
  5. Data show that a 121-nucleotide element within the 3'-untranslated region of hairy is necessary and sufficient to mediate apical transport. PMID: 12743042
  6. Hairy and Dl function concertedly and nonredundantly to define periodic proneural gene achaete expression. PMID: 16542648
  7. long-range effects may be mediated by a "spreading" mechanism, modifying chromatin over extensive regions to inhibit transcription PMID: 18285466
  8. These studies suggest that protein kinase CK2 contributes to repression by Hairy. PMID: 18601910
  9. hypoxia-selected flies: (a) altered their gene expression and genetic code, and (b) coordinated their metabolic suppression, especially during development, with hairy acting as a metabolic switch, thus playing a crucial role in hypoxia-tolerance PMID: 18927626

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Subcellular Location
Nucleus.
Database Links

KEGG: dme:Dmel_CG6494

STRING: 7227.FBpp0099504

UniGene: Dm.2554

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