Recombinant Drosophila melanogaster Protein held out wings (how)

Code CSB-YP517286DLU
MSDS
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Source Yeast
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Code CSB-EP517286DLU
MSDS
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Source E.coli
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Code CSB-EP517286DLU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP517286DLU
MSDS
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Source Baculovirus
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Code CSB-MP517286DLU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
how
Uniprot No.
Alternative Names
how; KH93F; qkr93F; stru; who; CG10293; Protein held out wings; KH domain protein KH93F; Protein muscle-specific; Protein struthio; Protein wings held out; Putative RNA-binding protein; Quaking-related 93F
Species
Drosophila melanogaster (Fruit fly)
Expression Region
1-405
Target Protein Sequence
MSVCESKAVV QQQLQQHLQQ QAAAAVVAVA QQQQAQAQAQ AQAQAQQQQQ APQVVVPMTP QHLTPQQQQQ STQSIADYLA QLLKDRKQLA AFPNVFTHVE RLLDEEIARV RASLFQINGV KKEPLTLPEP EGSVVTMNEK VYVPVREHPD FNFVGRILGP RGMTAKQLEQ ETGCKIMVRG KGSMRDKKKE DANRGKPNWE HLSDDLHVLI TVEDTENRAT VKLAQAVAEV QKLLVPQAEG EDELKKRQLM ELAIINGTYR DTTAKSVAVC DEEWRRLVAA SDSRLLTSTG LPGLAAQIRA PAAAPLGAPL ILNPRMTVPT TAASILSAQA APTAAFDQTG HGMIFAPYDY ANYAALAGNP LLTEYADHSV GAIKQQRRLA TNREHPYQRA TVGVPAKPAG FIEIQ
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Required for integrin-mediated cell-adhesion in wing blade. Vital role in steroid regulation of muscle development and to control heart rate. Required during embryogenesis, in late stages of somatic muscle development, for myotube migration and during metamorphosis for muscle reorganization.
Gene References into Functions
  1. Spatiotemporal regulation of HOW expression is required to specify the four spermatogonial transit-amplifying divisions. PMID: 20362539
  2. MAPK/ERK-dependent phosphorylation of HOW promotes the formation of HOW dimers and thus enhances its activity in controlling mRNA levels of key muscle-specific genes PMID: 22479211
  3. HOW has different roles in male and female Drosophila germline differentiation PMID: 22163028
  4. isoforms of the Drosophila RNA binding protein, how, act in opposing directions to regulate tendon cell differentiation PMID: 11832244
  5. Cell divisions in the drosophila embryonic mesoderm are repressed via posttranscriptional regulation of string/cdc25 by Held Out Wing protein. PMID: 15723789
  6. Four mRNAs were found to be specifically elevated in the mesoderm of how germline clone embryos, and to exhibit specific binding to HOW via their 3' UTRs. PMID: 17728343

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Subcellular Location
Nucleus.
Tissue Specificity
During embryogenesis, expression is seen in mesodermal precursors of somatic, visceral and pharyngeal muscle. Later in embryogenesis, expression is restricted to heart and muscle attachment sites of the epidermis. During onset of metamorphosis, expression
Database Links
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