Recombinant Drosophila melanogaster Protein lozenge (lz), partial

Code CSB-YP893836DLU
MSDS
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Source Yeast
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Code CSB-EP893836DLU
MSDS
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Source E.coli
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Code CSB-EP893836DLU-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP893836DLU
MSDS
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Source Baculovirus
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Code CSB-MP893836DLU
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
lz
Uniprot No.
Alternative Names
lz; CG1689; Protein lozenge
Species
Drosophila melanogaster (Fruit fly)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Involved in prepatterning photoreceptor precursors in the developing eye; in the larval eye disk it defines a subset of cells as an equipotential group that is competent to respond to the sevenless developmental signal and another subset that confer proper photoreceptor identity by positively regulating the homeo box gene Bar. Involved in the aop/pnt dynamic in a Ras-dependent manner to regulate pros expression. Promotes apoptosis in the pupal eye by directly activating aos and klu. Also modulates hid- and rpr-mediated cell death. Regulates amos function in olfactory sensilla development.
Gene References into Functions
  1. Identification of the Doublesex protein binding sites that activate expression of lozenge in the female genital disc in Drosophila melanogaster has been reported. PMID: 28087460
  2. Lz provides key intrinsic information to cells during the second wave of pattern formation in the developing ommatidium. PMID: 24067351
  3. mlf participates in a positive feedback loop to fine tune the activity of the RUNX transcription factor Lozenge (LZ) during development of the crystal cells. PMID: 22411814
  4. Data demonstrate that Med12 and Med13 but not CycC or Cdk8 are essential for Serpent/Lozenge-induced transactivation in cell culture. PMID: 20368357
  5. Results lead to conclude that Ttk69 can either directly or indirectly repress lz gene expression to prevent the premature development of R7 precursor cells in the developing eye of Drosophila. PMID: 20003234
  6. Yan regulates Lozenge during eye development PMID: 12111211
  7. Lozenge is required to contribute to the repression of cell death mechanisms, creating a permissive environment for the survival of undifferentiated cells in early eye development PMID: 12690448
  8. role in crystal cell and plasmatocyte production PMID: 14504400
  9. Lz can trigger crystal cell development. PMID: 14657024
  10. Lz promotes apoptosis in the eye during pupal development, in part by regulating argos and klumpfuss protein expression. PMID: 15879554
  11. Glial cells missing (GCM)/GCM2 play a key role in controlling the size of the crystal cell population by inhibiting lz activation and maintenance PMID: 16176949
  12. Required for serpent regulation of u-shaped. PMID: 16730345
  13. Srp and Lz do not just specify the crystal cell lineage, but also regulate the later differentiation of these cells. PMID: 17417793
  14. Maintenance of the crystal cell hematopoietic lineage choice and the realization of the crystal cell differentiation program rely on cooperation between RUNX factor Lozenge (Lz) and GATA factor Serpent (Srp). PMID: 17418114
  15. Results show that the GATA factor Serpent cross-regulates lozenge and u-shaped expression during Drosophila blood cell development. PMID: 17869239

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Subcellular Location
Nucleus.
Tissue Specificity
Expressed in the pupal eye during programmed cell death.
Database Links

KEGG: dme:Dmel_CG1689

STRING: 7227.FBpp0071255

UniGene: Dm.2929

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