Recombinant Drosophila melanogaster Protein mothers against dpp (Mad)

Code CSB-YP336953DLU
MSDS
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Source Yeast
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Code CSB-EP336953DLU
MSDS
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Source E.coli
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Code CSB-EP336953DLU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP336953DLU
MSDS
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Source Baculovirus
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Code CSB-MP336953DLU
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Mad
Uniprot No.
Alternative Names
Mad; CG12399Protein mothers against dpp
Species
Drosophila melanogaster (Fruit fly)
Expression Region
1-455
Target Protein Sequence
MDTDDVESNT SSAMSTLGSL FSFTSPAVKK LLGWKQGDEE EKWAEKAVDS LVKKLKKRKG AIEELERALS CPGQPSKCVT IPRSLDGRLQ VSHRKGLPHV IYCRVWRWPD LQSHHELKPL ELCQYPFSAK QKEVCINPYH YKRVESPVLP PVLVPRHSEF APGHSMLQFN HVAEPSMPHN VSYSNSGFNS HSLSTSNTSV GSPSSVNSNP NSPYDSLAGT PPPAYSPSED GNSNNPNDGG QLLDAQMGDV AQVSYSEPAF WASIAYYELN CRVGEVFHCN NNSVIVDGFT NPSNNSDRCC LGQLSNVNRN STIENTRRHI GKGVHLYYVT GEVYAECLSD SAIFVQSRNC NYHHGFHPST VCKIPPGCSL KIFNNQEFAQ LLSQSVNNGF EAVYELTKMC TIRMSFVKGW GAEYHRQDVT STPCWIEIHL HGPLQWLDKV LTQMGSPHNA ISSVS
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Required for the function of decapentaplegic. May play an important role in mediating Dpp signaling. Involved in the BMP signaling pathway.
Gene References into Functions
  1. LTR sequence of the MDG4 retrotransposon contains the MAD protein binding site that affects the east-dependent repression PMID: 28510129
  2. During development, synaptic pMad accumulation followed the arrival and clustering of ionotropic glutamate receptors at neuromuscular junction synapses. PMID: 24353060
  3. The actions of Brat at synapses are mediated through mothers against decapentaplegic (Mad), the signal transduction effector of the bone morphogenetic protein (BMP) signaling pathway. PMID: 23884941
  4. Data show that dSmad2 directly influences Baboon's phosphorylation of Mad. PMID: 22563507
  5. Mad has distinct signal transduction roles in the BMP and Wnt pathways depending on its phosphorylation state. PMID: 21990430
  6. Nup154 controls nuclear localization of Mad transcription factor, but does not interact directly with it. PMID: 21696798
  7. Yorkie and Mad physically bind each other, and 410 bp minimal enhancer of bantam that responds to Yorkie:Mad in vivo and in cultured cells, was identified. PMID: 21238929
  8. Heterodimers of SAX and TKV play an important role in extending the BMP activity gradient by facilitating DPP diffusion and assisting GBB signaling through functional complexes with type II receptors. PMID: 20381612
  9. importin-beta11 function interacts with the bone morphogenic protein pathway to regulate a pool of phosphorylated mothers against decapentaplegic that must be present at the presynapse for its proper development and function PMID: 20392948
  10. Mad depletion or overexpression produced Wingless-like embryonic segmentation phenotypes PMID: 19657393
  11. The downstream transcription factor Mothers against dpp is essential for the normal structural and functional development of the Drosophila neuromuscular junction, a synapse that displays activity-dependent plasticity. PMID: 12672013
  12. compared patterns of two related cellular responses, both signal-dependent phosphorylation of the BMP-regulated R-SMAD, MAD, and signal-dependent changes in levels and sub-cellular distribution of the co-SMAD Medea PMID: 14534137
  13. a complex of the homologous Drosophila Smad proteins, Mad and Medea, is capable of concerted binding to GC-rich and SBE sites separated by as much as 20 bp. PMID: 17610966
  14. A combinatorial enhancer recognized by Mad, TCF, and Brk first activates then represses dpp expression in the posterior spiracles of Drosophila. PMID: 18068697
  15. Results describe the crystal structure of the MH2 domain of Mad, which closely matches those of other Smad MH2 domains, is reported at 3.2 A resolution. PMID: 18997322
  16. phosphorylation of Mad by Nmo ensures normal accumulation and distribution of Mad and thereby fine tunes BMP signaling in motor neurons PMID: 19451277

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Subcellular Location
Cytoplasm. Nucleus.
Protein Families
Dwarfin/SMAD family
Tissue Specificity
Stage 13 embryos display expression in the dorsal epidermis (at protein level).
Database Links

KEGG: dme:Dmel_CG12399

STRING: 7227.FBpp0304648

UniGene: Dm.7367

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