Recombinant Drosophila melanogaster Protein phosphatase PP2A 55 kDa regulatory subunit (tws)

Code CSB-YP330653DLU
MSDS
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Source Yeast
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Code CSB-EP330653DLU
MSDS
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Source E.coli
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Code CSB-EP330653DLU-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP330653DLU
MSDS
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Source Baculovirus
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Code CSB-MP330653DLU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
tws
Uniprot No.
Alternative Names
tws; aar; Pp2A-85F; CG6235Protein phosphatase PP2A 55 kDa regulatory subunit; PR55; Protein phosphatase PP2A regulatory subunit B; Protein twins
Species
Drosophila melanogaster (Fruit fly)
Expression Region
1-499
Target Protein Sequence
MGRWGRQSPV LEPPDPQMQT TPPPPTLPPR TFMRQSSITK IGNMLNTAIN INGAKKPASN GEASWCFSQI KGALDDDVTD ADIISCVEFN HDGELLATGD KGGRVVIFQR DPASKAANPR RGEYNVYSTF QSHEPEFDYL KSLEIEEKIN KIRWLQQKNP VHFLLSTNDK TVKLWKVSER DKSFGGYNTK EENGLIRDPQ NVTALRVPSV KQIPLLVEAS PRRTFANAHT YHINSISVNS DQETFLSADD LRINLWHLEV VNQSYNIVDI KPTNMEELTE VITAAEFHPT ECNVFVYSSS KGTIRLCDMR SAALCDRHSK QFEEPENPTN RSFFSEIISS ISDVKLSNSG RYMISRDYLS IKVWDLHMET KPIETYPVHE YLRAKLCSLY ENDCIFDKFE CCWNGKDSSI MTGSYNNFFR VFDRNSKKDV TLEASRDIIK PKTVLKPRKV CTGGKRKKDE ISVDCLDFNK KILHTAWHPE ENIIAVAATN NLFIFQDKF
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Could perform a substrate recognition function or could be responsible for targeting the enzyme complex to the appropriate subcellular compartment.
Gene References into Functions
  1. Mutations in tws cause high frequencies of chromosome aberrations. Tws is required for activation of the G2/M DNA damage checkpoint. PMID: 28040742
  2. The PP2A complex plays a novel role in differentiating tissues to promote developmentally controlled quiescence through the regulation of Cyclin E/Cdk2 activity. PMID: 26253406
  3. Drosophila protein PP2A is a liprin-alpha-interacting protein that mediates interaction between the two proteins. PMID: 24948803
  4. E4orf4 has a role in inducing PP2A- and Src-dependent cell death while inhibiting classic apoptosis pathways in Drosophila melanogaster PMID: 23613593
  5. Greatwall and Endos act to promote the inactivation of PP2A-Twins/B55 in Drosophila PMID: 21852956
  6. PP2A-B' knockout flies have elevated S6K phosphorylation and exhibit phenotypes typical of elevated insulin signaling such as reduced total body triglycerides and reduced longevity. PMID: 20444422
  7. Data demonstrate that PP2A is required for Wnt/beta-catenin signaling in Drosophila. PMID: 19556239

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Protein Families
Phosphatase 2A regulatory subunit B family
Database Links

KEGG: dme:Dmel_CG6235

STRING: 7227.FBpp0081665

UniGene: Dm.3826

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