Recombinant Drosophila melanogaster Protein prickle (pk), partial

Code CSB-YP381174DLU
MSDS
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Source Yeast
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Code CSB-EP381174DLU
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Source E.coli
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Code CSB-EP381174DLU-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP381174DLU
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Source Baculovirus
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Code CSB-MP381174DLU
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
pk
Uniprot No.
Alternative Names
pk; CG11084; Protein prickle; Protein spiny legs
Species
Drosophila melanogaster (Fruit fly)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Acts in a planar cell polarity (PCP) complex; polarization along the apical/basal axis of epithelial cells. Correct expression of the alternative isoforms is required for PCP signaling in imaginal disks. PCP signaling in the wing disk requires the receptor fz and the cytoplasmic proteins dsh and pk. These act in a feedback loop leading to activation of the jnk cascade and subsequent polarized arrangement of hairs and bristles. Dgo and pk compete with one another for dsh binding, thereby modulating fz dsh activity and ensuring tight control over fz PCP signaling. Vang, stan and pk function together to regulate the establishment of tissue polarity in the adult eye.
Gene References into Functions
  1. We find that the E3 ubiquitin ligase complex Cullin1(Cul1)/SkpA/Supernumerary limbs(Slimb) regulates the stability of one of the peripheral membrane components, Prickle (Pk). PMID: 25996914
  2. Pk and Sple form different complexes with distinct subcellular localizations in the eye and wing. PMID: 24998533
  3. Fat and Core planar cell polarity (PCP) pathways patterns change during morphogenesis, undergoing phases of coupling and uncoupling that are regulated by antagonistic Core PCP protein isoforms Prickle and Spiny-legs. PMID: 25201685
  4. These data reveal a previously unidentified pathway in the pathophysiology of seizure disorders and provide evidence for a more generalized cellular mechanism whereby Prickle mediates polarity by influencing microtubule-mediated transport. PMID: 25024231
  5. Alternative Prickle (Pk-Sple) protein isoforms control the polarity of microtubule network. PMID: 25005476
  6. Pk mediates feedback amplification to generate asymmetric planar cell polarity signaling in Drosophila. PMID: 12015986
  7. Prickle localization is influenced by Strabismus function. PMID: 12642492
  8. the initial membrane association of Diego depends on Fz, and that Diego physically interacts with Strabismus and Pk through its Ankyrin repeats PMID: 15306567
  9. Prickle competitively binds with Dishevelled and thereby modulating Frizzled/Dishevelled activity. PMID: 15937478
  10. Pk(Pk) is sufficient to mediate the intercellular feedback signalling. Significantly, Pk(Pk) but not Pk(Sple) is required for hexagonal cell packing in the pupal wing. PMID: 19028485
  11. analysis of structural and membrane binding properties of the prickle PET domain PMID: 19053268

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Subcellular Location
Cell membrane; Peripheral membrane protein; Cytoplasmic side.
Protein Families
Prickle / espinas / testin family
Tissue Specificity
Expressed in the wing, leg and eye imaginal disks. Expressed within the photoreceptors of the eye.
Database Links

KEGG: dme:Dmel_CG11084

STRING: 7227.FBpp0088115

UniGene: Dm.3470

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