Recombinant Drosophila melanogaster Protein pygopus (pygo), partial

Code CSB-YP895583DLU
MSDS
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Source Yeast
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Code CSB-EP895583DLU
MSDS
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Source E.coli
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Code CSB-EP895583DLU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP895583DLU
MSDS
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Source Baculovirus
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Code CSB-MP895583DLU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
pygo
Uniprot No.
Alternative Names
pygo; gam; CG11518; Protein pygopus; Protein gammy legs
Species
Drosophila melanogaster (Fruit fly)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Involved in signal transduction through the Wnt pathway.
Gene References into Functions
  1. results are consistent with the idea that pygo functions independently of canonical Wnt signaling in the differentiation of the adult interchamber cardiac valves PMID: 24265259
  2. humanized fly Pygo derepresses Notch targets, implying an inherent Notch-related function of classical Pygo orthologs, disabled in fly Pygo, which thus appears dedicated to Wnt signaling. PMID: 24183574
  3. Double knockdown of pygo and Ca2+/calmodulin-dependent protein kinase II caused additional arrhythmia compared with pygo knockdown alone, suggesting that some of the effects of pygo are mediated by Ca2+ signaling. PMID: 24046329
  4. nuclear component of the Wnt signalling pathway; presence of a PHD domain implicates Pygo protein in a chromatin-related function PMID: 11988739
  5. nuclear PHD-finger protein required for Wingless signaling in Drosophila. PMID: 12015286
  6. identifed conserved residues within Pygopus that are required for binding to Legless in vitro and for their activity in development PMID: 14612447
  7. Pygopus and legless provide essential transcriptional coactivator functions to armadillo/beta-catenin. PMID: 16005293
  8. Data show that human and Drosophila Pygo PHD fingers associate with their cognate HD1 domains from BCL9/Legless to bind specifically to the histone H3 tail methylated at lysine 4 (H3K4me). PMID: 18498752
  9. Pygo predisposes Drosophila T cell factor target genes for rapid Wingless-induced transcription, or it protects them against premature shut-down PMID: 19036929
  10. the PHD domain is required to link Drosophila Pygopus to Legless/beta-catenin and not to histone H3 PMID: 19493659

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Subcellular Location
Nucleus.
Tissue Specificity
Ubiquitous throughout embryogenesis and larval development.
Database Links

KEGG: dme:Dmel_CG11518

STRING: 7227.FBpp0085167

UniGene: Dm.5270

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