Recombinant Drosophila melanogaster Protein singed (sn), partial

Code CSB-YP630513DLU
MSDS
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Source Yeast
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Code CSB-EP630513DLU
MSDS
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Source E.coli
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Code CSB-EP630513DLU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP630513DLU
MSDS
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Source Baculovirus
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Code CSB-MP630513DLU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
sn
Uniprot No.
Alternative Names
sn; CG1536; Protein singed
Species
Drosophila melanogaster (Fruit fly)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Acts as an actin bundling protein. May have a role in the asymmetric organization and/or movement of cytoplasmic components. It has a role in somatic cells during the formation of adult bristles and hairs, and in the female germline during oogenesis.
Gene References into Functions
  1. Alterations in nuclear Fascin levels result in defects in nucleolar morphology in both Drosophila follicles and cultured mammalian cells, suggesting that nuclear Fascin plays an important role in nucleolar architecture. PMID: 25808493
  2. essential for actin bundle initiation and stiffening during embryonic tracheal system development PMID: 25482727
  3. Fascin and Ena cooperate to extend and maintain robust filopodia of uniform thickness with aligned barbed ends by a unique mechanistic cycle. PMID: 24591594
  4. Fascin links Btl/FGFR signalling to the actin cytoskeleton during Drosophila tracheal morphogenesis. PMID: 24496629
  5. These data lead to the conclusion that prostaglandins regulate Fascin to control actin remodeling PMID: 23051736
  6. The terminal branchlets of different classes of neurons have distinctive dynamics and are formed on the basis of molecularly separable mechanisms; in particular, class III neurons require fascin for terminal branching whereas class IV neurons do not. PMID: 22764047
  7. The regulatory role of FascinS52 appears to be tissuespecific, and its importance might depend on the dynamics and the turnover rate of actin bundles between various cell types. PMID: 19901519
  8. Fascin deficiency does not impair 20-hydroxyecdysone response, but neurites fail to maintain their normal, nearly straight trajectory, instead forming curls and hooks. This is accompanied by abnormally distributed filamentous actin. PMID: 16928862
  9. Fascin is required for plasmatocyte migration, both as these cells undergo developmental dispersal and during an inflammatory response to epithelial wounding. PMID: 19592575
  10. study found that Rab35 regulates the assembly of actin filaments during bristle development; effect was mediated by the actin-bundling protein fascin, which directly associated with active Rab35 PMID: 19729655

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Subcellular Location
Cytoplasm, cytoskeleton.
Protein Families
Fascin family
Database Links

KEGG: dme:Dmel_CG32858

STRING: 7227.FBpp0290893

UniGene: Dm.7274

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