Recombinant Drosophila melanogaster Protein snail (sna)

Code CSB-YP357284DLU
MSDS
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Source Yeast
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Code CSB-EP357284DLU
MSDS
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Source E.coli
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Code CSB-EP357284DLU-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP357284DLU
MSDS
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Source Baculovirus
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Code CSB-MP357284DLU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
sna
Uniprot No.
Alternative Names
sna; CG3956Protein snail
Species
Drosophila melanogaster (Fruit fly)
Expression Region
1-390
Target Protein Sequence
MAANYKSCPL KKRPIVFVEE RLPQTEALAL TKDSQFAQDQ PQDLSLKRGR DEETQDYQQP EPKRDYVLNL SKTPERNSSS SSNSCLLSPP VEAQDYLPTE IHMRGLTAGT TGYTTATPTT INPFQSAFVM AAGCNPISAL WSSYQPHLAA FPSPASSMAS PQSVYSYQQM TPPSSPGSDL ETGSEPEDLS VRNDIPLPAL FHLFDEAKSS SSGASVSSSS GYSYTPAMSA SSASVAANHA KNYRFKCDEC QKMYSTSMGL SKHRQFHCPA AECNQEKKTH SCEECGKLYT TIGALKMHIR THTLPCKCPI CGKAFSRPWL LQGHIRTHTG EKPFQCPDCP RSFADRSNLR AHQQTHVDVK KYACQVCHKS FSRMSLLNKH SSSNCTITIA
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Essential for the correct specification of ventral-dorsal patterns.
Gene References into Functions
  1. While either Snail or Serpent induced a profound loss of epithelial polarity and tissue organisation, Serpent but not Snail also induced an increase in the size of wing discs. Furthermore, the Serpent-induced tumour-like tissues were able to grow extensively when transplanted into the abdomen of adult hosts. PMID: 29420531
  2. Disruption of Snail expression in follicle stem cells compromises proliferation, but not maintenance. FSCs with excessive Snail expression had increased proliferation and lifespan, accompanied by a moderate decrease inE-cadherin expression (required for adhesion of FSCs to their niche) at the junction between their adjacent cells, indicating a conserved role of Snail in E-cadherin inhibition. PMID: 27141871
  3. during gastrulation of Drosophila embryos, Sna expression downregulates polarity protein Baz which in turn results in junction disassembly at protein levels. PMID: 28063874
  4. Snail can potentiate enhancer activation by collaborating with different activators, providing a new mechanism by which Snail regulates development. PMID: 24402316
  5. Rapid transcription kinetics and negative autoregulation are responsible for the remarkable homogeneity of snail expression and the coordination of mesoderm invagination. PMID: 23352665
  6. Study shows that Sna represses transcription of pbl in the mesoderm primordium of D. melanogaster via one or more Sna-binding sites, which are conserved among species of the Drosophila genus, but not in the mosquito, correlating with the different modes of gastrulation in the different genuses. PMID: 22945369
  7. Complex interactions between cis-regulatory modules in native conformation are critical for Drosophila snail expression. PMID: 21813571
  8. Dorsal activates twist and snail, and the Dorsal/Twist/Snail network activates and represses other zygotic genes to form the dorsoventral axis in Drosophila. PMID: 15987775
  9. Snail represses Tom expression in the mesoderm and thereby activates Delta trafficking. PMID: 16459304

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Subcellular Location
Nucleus.
Protein Families
Snail C2H2-type zinc-finger protein family
Database Links

KEGG: dme:Dmel_CG3956

STRING: 7227.FBpp0080298

UniGene: Dm.3035

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