Recombinant Drosophila melanogaster Protein twist (twi)

Code CSB-YP318774DLU
MSDS
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Source Yeast
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Code CSB-EP318774DLU
MSDS
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Source E.coli
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Code CSB-EP318774DLU-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP318774DLU
MSDS
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Source Baculovirus
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Code CSB-MP318774DLU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
twi
Uniprot No.
Alternative Names
twi; CG2956; Protein twist
Species
Drosophila melanogaster (Fruit fly)
Expression Region
1-490
Target Protein Sequence
MMSARSVSPK VLLDISYKPT LPNIMELQNN VIKLIQVEQQ AYMQSGYQLQ HQQQHLHSHQ HHQQHHQQQH AQYAPLPSEY AAYGITELED TDYNIPSNEV LSTSSNQSAQ STSLELNNNN TSSNTNSSGN NPSGFDGQAS SGSSWNEHGK RARSSGDYDC QTGGSLVMQP EHKKLIHQQQ QQQQQHQQQI YVDYLPTTVD EVASAQSCPG VQSTCTSPQS HFDFPDEELP EHKAQVFLPL YNNQQQQSQQ LQQQQPHQQS HAQMHFQNAY RQSFEGYEPA NSLNGSAYSS SDRDDMEYAR HNALSSVSDL NGGVMSPACL ADDGSAGSLL DGSDAGGKAF RKPRRRLKRK PSKTEETDEF SNQRVMANVR ERQRTQSLND AFKSLQQIIP TLPSDKLSKI QTLKLATRYI DFLCRMLSSS DISLLKALEA QGSPSAYGSA SSLLSAAANG AEADLKCLRK ANGAPIIPPE KLSYLFGVWR MEGDAQHQKA
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Involved in the establishment and dorsoventral patterning of germ layers in the embryo.
Gene References into Functions
  1. The level of Twi activity determines whether the cellular events of ventral furrow formation, epithelial-to-mesenchymal transition, cell division and mesodermal migration occur. PMID: 24915423
  2. Our studies identify Akirin as a nuclear factor that genetically interacts with the BRM complex and is required for optimal expression of the Twist-dependent Dmef2 enhancer PMID: 22396663
  3. conclude that Twi is an essential Notch co-regulator in myogenic progenitor cells and has the potential to confer specificity on Notch signalling at over 170 genes, showing that a single factor can have a profound effect on the output of the pathway. PMID: 20610485
  4. The role of Twist in the Toll/Dorsal signaling pathway that specifies patterning thresholds of gene expression PMID: 12091311
  5. anterior-gut formation is mechanically induced by the movement of germ-band extension through the induction of Twist expression in stomodeal cells PMID: 12932320
  6. High Twist levels direct cells to the body wall muscle fate, whereas low levels are permissive for gut muscle and fat body fate. PMID: 15128668
  7. twi expression in Drosophila during gastrulation and mesoderm formation was compared to twi expression in the same embryonic development stages in Tribolium castaneum. PMID: 15645317
  8. Twist binds to almost all mesodermal cis-regulatory modules to provide the competence to integrate inputs from more specialized transcription factors. PMID: 17322403
  9. These experiments show that mechanically induced Twist upregulation in stomodeal cells is necessary for subsequent midgut differentiation in Drosophila embryos. PMID: 18804441
  10. Data show that cell fate decisions in the dorsal and lateral Twi- and Tin-positive mesoderm of Drosophila melanogaster depend on the antagonistic action of the transcription factor Lame duck and the zinc finger homeodomain factor Zfh1. PMID: 19028484

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Subcellular Location
Nucleus.
Tissue Specificity
Expressed in embryonic abdomen; a single cell ventrally, pairs of cells laterally and three cells dorsally in each hemisegment. In the thorax, there are patches of cells associated with the imaginal disks. During larval development, cells proliferate and,
Database Links

KEGG: dme:Dmel_CG2956

STRING: 7227.FBpp0071864

UniGene: Dm.5605

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