Recombinant Drosophila melanogaster Ras-like GTP-binding protein Rho1 (Rho1)

Code CSB-YP343711DLU
MSDS
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Source Yeast
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Code CSB-EP343711DLU
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Source E.coli
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Code CSB-EP343711DLU-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP343711DLU
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Source Baculovirus
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Code CSB-MP343711DLU
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Rho1
Uniprot No.
Alternative Names
Rho1; CG8416; Ras-like GTP-binding protein Rho1
Species
Drosophila melanogaster (Fruit fly)
Expression Region
1-189
Target Protein Sequence
MTTIRKKLVI VGDGACGKTC LLIVFSKDQF PEVYVPTVFE NYVADIEVDG KQVELALWDT AGQEDYDRLR PLSYPDTDVI LMCFSVDSPD SLENIPEKWT PEVKHFCPNV PIILVGNKKD LRNDPNTIRD LAKMKQEPVK PQEGRAMAEK INAFAYLECS AKSKEGVRDV FETATRAALQ VKKRKKTRC
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Has a role in regulating actin cytoskeletal organization: required during early development for proper execution of morphogenetic movements of individual cells and groups of cells important for the formation of the embryonic body plan. Plays a role in regulating dorsal closure during embryogenesis. During axis elongation, required for Rho-kinase Rok planar polarity and adherens junction localization as well as for generating a planar polarized distribution of the actin-binding protein Shrm. During embryogenesis, acts upstream of wash to regulate the developmental migration of tail hemocytes anteriorly along the ventral midline. May have a role in eye development.
Gene References into Functions
  1. Study identified circadian rhythms in Rho1 GTPase activity in s-LNv axons that are regulated by rhythmic transcription of Pura, a Rho1 GEF. Pura activates Rho1 to retract s-LNv axons, decrease active zone numbers and reduce s-LNvs' influence on the clock network. Thus, study links transcription, plasticity, network hierarchy and behavior. PMID: 26234154
  2. As tension in the epithelial layer increases, Rho kinase signaling activates myosin assembly and contraction in the developing Drosophila egg chamber. PMID: 24943847
  3. This work identifies spatially distinct functions for Rho1 in the regulation of DE-cadherin-containing vesicular trafficking during adherens junctions remodeling in live epithelia. PMID: 25079692
  4. impaired Hippo signaling induces JNK activation through Rho1. PMID: 25583514
  5. Galpha73B is a downstream effector of JAK/STAT signaling and a regulator of Rho1 in Drosophila hematopoiesis. PMID: 24163435
  6. The RhoA fails to form a compact ring in late cytokinesis after Sti depletion, and this function requires Sti kinase activity. PMID: 22084308
  7. removal of a single copy of rho1 can suppress the pak phenotype PMID: 21098722
  8. Results suggest that Crossveinless-c contributes to sprouting and subsequent growth of the anterior-posterior-oriented branches through negative regulation of Rho1. PMID: 20384791
  9. Continuous autophagy was required for integrin-mediated hemocyte spreading and Rho1-induced cell protrusions. PMID: 20498061
  10. disruption of Rho1 suppresses apical constriction and invagination in Adenomatous polyposis coli null cells PMID: 20102708
  11. Results suggest that Rho1 functions at the cell cortex to regulate JNK activity and implicate Rho1 and moesin in epithelial cell survival. PMID: 20404112
  12. analysis of second-site noncomplementation screen for modifiers of Rho1 signaling during imaginal disc morphogenesis in Drosophila PMID: 19862331
  13. Drosophila RhoA regulates the cytoskeleton and cell-cell adhesion in the developing epidermis. PMID: 12070092
  14. While Rho1 protein is present throughout the cell, it accumulates apically, particularly at sites of cadherin-based adherens junctions. PMID: 12135916
  15. RhoA and Sb-sbd act in a common pathway during leg morphogenesis PMID: 14668391
  16. role for Rho1 in regulating signaling events governing proper patterning during development PMID: 15649467
  17. Taken together, these findings suggest that RhoGEF4 may participate in cytoskeleton-related cellular events by specifically activating RhoA in neuronal morphogenesis. PMID: 17011730
  18. Reducing activity of Rho1 from somatic support cells suppressed the germ cell enclosure defects of the conditional Spitz allele. PMID: 17629483
  19. In parallel to regulation of crb RNA and protein, Rho1 activity also signals through Rho-kinase (Rok) to induce apical constriction and cell shape change during invagination of salivary gland. PMID: 18585373
  20. Results demonstrate that Rho1 is required to maintain adherens junction integrity independent of its role in sustaining apical cell tension. PMID: 19506041
  21. in addition to its interaction with Mwh Rho1 has functions in wing planar polarity that are parallel to and upstream of fz. PMID: 19576201

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Subcellular Location
Cell membrane; Lipid-anchor; Cytoplasmic side. Cytoplasm, cytoskeleton. Apical cell membrane. Lateral cell membrane.
Protein Families
Small GTPase superfamily, Rho family
Tissue Specificity
Expressed in hemocytes (at protein level).
Database Links

KEGG: dme:Dmel_CG8416

STRING: 7227.FBpp0086354

UniGene: Dm.644

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