Recombinant Drosophila melanogaster Segmentation polarity homeobox protein engrailed (en), partial

Code CSB-YP360890DLU
MSDS
Size Pls inquire
Source Yeast
Have Questions? Leave a Message or Start an on-line Chat
Code CSB-EP360890DLU
MSDS
Size Pls inquire
Source E.coli
Have Questions? Leave a Message or Start an on-line Chat
Code CSB-EP360890DLU-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
Have Questions? Leave a Message or Start an on-line Chat
Code CSB-BP360890DLU
MSDS
Size Pls inquire
Source Baculovirus
Have Questions? Leave a Message or Start an on-line Chat
Code CSB-MP360890DLU
MSDS
Size Pls inquire
Source Mammalian cell
Have Questions? Leave a Message or Start an on-line Chat

Product Details

Purity
>85% (SDS-PAGE)
Target Names
en
Uniprot No.
Alternative Names
en; CG9015; Segmentation polarity homeobox protein engrailed
Species
Drosophila melanogaster (Fruit fly)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

 Customer Reviews

There are currently no reviews for this product.

Submit a Review here

Target Background

Function
This protein specifies the body segmentation pattern. It is required for the development of the central nervous system. Transcriptional regulator that represses activated promoters. Wg signaling operates by inactivating the SGG repression of EN autoactivation.
Gene References into Functions
  1. Data show that the Distalless and Engrailed homeodomain transcription factors act through sequences in this enhancer to establish elevated Sex combs reduced (Scr) expression in spatially defined domains. PMID: 29634724
  2. these results suggest that En in Johnston's Organ neurons regulate both neuronal excitability and synaptic connectivity. PMID: 25164665
  3. Silencing Engrailedin Johnston's organ neurons reduces the sound-evoked potential amplitude at the onset of the stimulus by about half at 100, 200 and 400 Hz. PMID: 23940751
  4. This study showed that small subset of FRUM neurons distributed in the brain and ventral cord of males by their co-expression with in En neurons PMID: 23688386
  5. Our studies identified several abnormal phenotypes in live engrailed (en) mutant embryos prior to cycle 8. Nuclei in en embryos divide asynchronously, an abnormality that was detected as early as nuclear cycle 2-3. PMID: 23593026
  6. Polycomb group protein binding per se does not determine the transcriptional state of engrailed. PMID: 23139817
  7. During development of the Drosophila ocellar complex, engrailed (en) plays a role both as a target and as a transcriptional repressor of hedgehog (hh) signaling in the prospective interocellar region. PMID: 23154412
  8. Anterior crossvein formation requires En function. An extracellular pool of En protein is present in the wing disc that diffuses beyond its expression domain and is required for the formation of the anterior crossvein. PMID: 21558379
  9. Data suggests that engrailed folds to its native structure via an intermediate in which the HTH motif is formed, and that secondary structure development occurs first followed by tertiary packing. PMID: 21035367
  10. Role of en and novel interactions between msh, ind, and vnd in dorsoventral patterning of the Drosophila brain and ventral nerve cord PMID: 20673828
  11. high levels of Engrailed make the epidermal cells inhospitable to bristle neurons; sensory axons that are too near these cells are either deflected or fail to extend properly or at all PMID: 20667917
  12. electrostatics significantly affect the stability of designed homeodomain variants PMID: 11829512
  13. relationship between engrailed and dominant additional vein mutant PMID: 12502252
  14. En is a bifunctional transcription factor and the recruitment of additional cofactors presumably specifies which function prevails on an individual promoter. PMID: 12506003
  15. Folding pathway of the three-helix bundle protein Engrailed homeodomain PMID: 12594518
  16. crystal structures of engrailed homeodomain mutants PMID: 12923178
  17. Engrailed is responsible for the establishment of ci repression early during embryonic development and is also required to maintain the repression of ci throughout development. PMID: 15282166
  18. Engrailed's function during the segregation of the neuroblasts is crucial for regulating different actors that are later involved in axon guidance PMID: 17126316
  19. compartmental expression of Ubx and abd-A is established through a repressive regulatory network between en, slp, Ubx and abd-A PMID: 17573068
  20. Engrailed Polycomb-group response elements bring chromatin together, allowing both positive and negative regulatory interactions between distantly located DNA fragments. PMID: 18199580
  21. Molecular dynamics simulations were performed on an ultrafast unfolding and folding protein at its melting temperature to observe, on an atom-by-atom basis, the pathways the protein followed as it unfolded and folded within a continuous trajectory. PMID: 18553935
  22. Engrailed expression in subsets of adult Drosophila sensory neurons. PMID: 18597129
  23. Using a Drosophila model of Huntington's disease, we found that Engrailed (EN), a transcriptional activator of endogenous Drosophila htt (dhtt), is able to prevent aggregation of polyQ-hHtt. PMID: 18718937
  24. en expression is down-regulated in the posterior portion of wing discs, indicating that the PRC2 complex acts as an activator of en. PMID: 19368801
  25. Data show that engrailed enhancers can act over large distances, even skipping over other transcription units, choosing the engrailed promoter over those of neighboring genes. PMID: 19675130

Show More

Hide All

Subcellular Location
Nucleus.
Protein Families
Engrailed homeobox family
Database Links

KEGG: dme:Dmel_CG9015

STRING: 7227.FBpp0087197

UniGene: Dm.22056

CUSABIO guaranteed quality
icon of phone
Call us
301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
icon of address
Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
icon of social media
Join us with

Subscribe newsletter

Leave a message

* To protect against spam, please pass the CAPTCHA test below.
CAPTCHA verification
© 2007-2024 CUSABIO TECHNOLOGY LLC All rights reserved. 鄂ICP备15011166号-1