Recombinant Drosophila melanogaster Semaphorin-2A (Sema-2a), partial

Code CSB-YP638761DLU
MSDS
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Source Yeast
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Code CSB-EP638761DLU
MSDS
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Source E.coli
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Code CSB-EP638761DLU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP638761DLU
MSDS
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Source Baculovirus
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Code CSB-MP638761DLU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Sema2a
Uniprot No.
Alternative Names
Sema2a; Sema-2a; CG4700; Semaphorin-2A; Semaphorin-II; Sema II
Species
Drosophila melanogaster (Fruit fly)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Plays a role in growth cones guidance. Required for both proper adult behavior and survival. Can function in vivo as a selective target-derived signal that inhibits the formation of specific synaptic terminal arbors.
Gene References into Functions
  1. PlexB is a receptor for the secreted semaphorin Sema-2a PMID: 16672342
  2. These data support a model in which Sema2a, while being secreted from oenocytes, represses axon extension from the sensory neuron cell body, but only in regions in direct contact with oenocytes. PMID: 17109838
Subcellular Location
Secreted.
Protein Families
Semaphorin family
Tissue Specificity
Transiently expressed by a single large muscle during motoneuron outgrowth and synapse formation.
Database Links

KEGG: dme:Dmel_CG4700

STRING: 7227.FBpp0086237

UniGene: Dm.14046

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