Recombinant Drosophila melanogaster Serine protease easter (ea)

Code CSB-YP321130DLU
MSDS
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Source Yeast
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Code CSB-EP321130DLU
MSDS
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Source E.coli
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Code CSB-EP321130DLU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP321130DLU
MSDS
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Source Baculovirus
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Code CSB-MP321130DLU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
ea
Uniprot No.
Alternative Names
ea; CG4920Serine protease easter; EC 3.4.21.-
Species
Drosophila melanogaster (Fruit fly)
Expression Region
128-392
Target Protein Sequence
IYG GMKTKIDEFP WMALIEYTKS QGKKGHHCGG SLISTRYVIT ASHCVNGKAL PTDWRLSGVR LGEWDTNTNP DCEVDVRGMK DCAPPHLDVP VERTIPHPDY IPASKNQVND IALLRLAQQV EYTDFVRPIC LPLDVNLRSA TFDGITMDVA GWGKTEQLSA SNLKLKAAVE GSRMDECQNV YSSQDILLED TQMCAGGKEG VDSCRGDSGG PLIGLDTNKV NTYYFLAGVV SFGPTPCGLA GWPGVYTLVG KYVDWIQNTI ES
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Component of the extracellular signaling pathway that establishes the dorsal-ventral pathway of the embryo. Three proteases; ndl, gd and snk process easter to create active easter. Active easter defines cell identities along the dorsal-ventral continuum by activating the spz ligand for the Tl receptor in the ventral region of the embryo.
Gene References into Functions
  1. The study reports the expression and refolding of Easter, and show that the renatured enzyme performs the activation cleavage of two spatzle isoforms. PMID: 23729565
  2. Easter plays a role in generating the wild-type shape of the Dorsal gradient in the developing Drosophila embryo PMID: 12421704
Subcellular Location
Secreted.
Protein Families
Peptidase S1 family, CLIP subfamily
Database Links

KEGG: dme:Dmel_CG4920

STRING: 7227.FBpp0082539

UniGene: Dm.2421

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