Recombinant Drosophila melanogaster Serine/threonine-protein kinase Warts(wts) ,partial

Code CSB-YP895584DLU
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Source Yeast
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Code CSB-EP895584DLU
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Source E.coli
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Code CSB-EP895584DLU-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP895584DLU
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Source Baculovirus
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Code CSB-MP895584DLU
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Source Mammalian cell
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Product Details

Purity >85% (SDS-PAGE)
Target Names wts
Uniprot No. Q9VA38
Alternative Names wts; CG12072; Serine/threonine-protein kinase Warts; EC 2.7.11.1
Species Drosophila melanogaster (Fruit fly)
Protein Length Partial
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

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Target Background

Function
Negative regulator of Yorkie (Yki) in the Hippo/SWH (Sav/Wts/Hpo) signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein Hippo (Hpo), in complex with its regulatory protein Salvador (Sav), phosphorylates and activates Warts (Wts) in complex with its regulatory protein Mats, which in turn phosphorylates and inactivates the Yorkie (Yki) oncoprotein. The Hippo/SWH signaling pathway inhibits the activity of the transcriptional complex formed by Scalloped (sd) and Yki and the target genes of this pathway include cyclin-E (cycE), diap1 and bantam. Inhibits nuclear localization of Yki. Regulates salivary gland degradation in a PI3K-dependent manner and Yki- and Sd-independent, mechanism.
Gene References into Functions
  1. Results demonstrate distinct lipid metabolism defects elicited in hypoxia as well as a novel role for insulin signaling and Warts function in the adaptation to low oxygen availability. PMID: 25541690
  2. In the normal epithelium, JNK signaling antagonizes the Hippo pathway effector Yorkie (Yki) through elevation of Warts activity, thereby suppressing tissue growth. In contrast, in the presence of hyperactive Ras, JNK signaling enhances Yki activation by accumulating F-actin through the activity of the LIM domain protein Ajuba, thereby promoting tissue growth. PMID: 25967126
  3. The Warts pathway restrains tissue growth by phosphorylating and inactivating the oncoprotein Yorkie. PMID: 22075147
  4. The warts protein pathway restrains tissue growth by phosphorylating and inactivating the oncoprotein Yorkie. PMID: 22075148
  5. Drosophila wts mutations are recessive lethal and homozygous clones in heterozygotes lead to epithelial tumors PMID: 12489118
  6. Data report the identification of Hippo, which regulates both cell proliferation and cell death in Drosophila and phosphorylates the tumor suppressor protein Salvador, which interacts with the Warts protein kinase. PMID: 12941273
  7. Data show that Hippo links Salvador and Warts to a key regulator of apoptosis PMID: 12941274
  8. Results suggest that Hippo acts together with Salvador and Warts in a signalling module that coordinately regulates cell proliferation and apoptosis. PMID: 14502294
  9. Data show that warts genes are dispensable in germline cells for their proliferation control. PMID: 18095349
  10. Wts may be regulated by distinct junction inputs depending on developmental context. PMID: 18430928
  11. regulates salivary gland cell death in a PI3K-dependent manner PMID: 18818081

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Subcellular Location Cytoplasm, cytosol. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Note=Colocalizes with mats and cyclin E at the centrosome.
Protein Families Protein kinase superfamily, AGC Ser/Thr protein kinase family
Database Links

KEGG: dme:Dmel_CG12072

STRING: 7227.FBpp0085082

UniGene: Dm.4172

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