Recombinant Drosophila melanogaster Serine/threonine-protein kinase tricorner (trc)

Code CSB-YP882036DLU
MSDS
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Source Yeast
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Code CSB-EP882036DLU
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Source E.coli
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Code CSB-EP882036DLU-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP882036DLU
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Source Baculovirus
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Code CSB-MP882036DLU
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
trc
Uniprot No.
Alternative Names
trc; CG8637; Serine/threonine-protein kinase tricorner; EC 2.7.11.1; NDR protein kinase; Serine/threonine-protein kinase 38-like
Species
Drosophila melanogaster (Fruit fly)
Expression Region
1-463
Target Protein Sequence
MMSSRTQDAD GASIRFSDHT LDKATKAKVT LENYYSNLVT QYGERKQRLA KLEAQLKDES LSEAQRQEKR LQHAQKETEY LRLKRLRLGV EDFEALKVIG RGAFGEVRLV QKKDTGHVYA MKVLRKADML EKEQVAHVRA ERDVLVEADH QWVVKMYYSF QDPVNLYLIM EFLPGGDMMT LLMKKDTLSE EGTQFYISET ALAIDSIHKL GFIHRDIKPD NLLLDARGHL KLSDFGLCTG LKKSHRTDFY RDLSQAKPSD FIGTCASLSC SPMDSKRRAE SWKRNRRALA YSTVGTPDYI APEVFLQTGY GPACDWWSLG VIMYEMLMGY PPFCSDNPQD TYRKVMNWRE TLIFPPEIPI SEEAKETIIN FCCEADRRLG SQRGLEDLKS VPFFRGVDWE HIRERPAAIP VEVRSIDDTS NFDEFPDVSL EIPSAPIPQG GEIAKDWVFI NYTYKRFEVR NLE
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Serine/threonine-protein kinase involved in controlling cell structure and proliferation of a variety of polarized outgrowths including epidermal hairs, bristles, arista laterals, and dendrites. Together with fry, maintains the integrity of epidermal hairs and is an essential component of the signaling pathway regulating dendritic branching of sensory neurons. Reduces neurite outgrowth by phosphorylating pav, thereby inhibiting its function in microtubule-microtubule sliding.
Gene References into Functions
  1. Data show that Tricornered Kinase (Trc) regulates the Wiskott-Aldrich syndrome protein (WASP) levels largely, by regulating the transcription of WASP. PMID: 26393506
  2. The regulation of Warts conformation by Mats, Fat/Dachsous signaling, and Dachs appears independent of Warts phosphorylation by Hippo kinase, establishing a precedent for the control of Nuclear Dbf2-related kinases PMID: 26702832
  3. Mutations in trc and wts genes lead to changes in cell shape, cellular levels of F-actin, the timing of differentiation, and the expression of multiple wing hairs and DE-Cadherin. PMID: 20211163
Subcellular Location
Cytoplasm. Nucleus.
Protein Families
Protein kinase superfamily, AGC Ser/Thr protein kinase family
Tissue Specificity
Expressed in the peripheral and central nervous system (at protein level). Expressed in the wing imaginal disk.
Database Links

KEGG: dme:Dmel_CG8637

STRING: 7227.FBpp0304464

UniGene: Dm.2518

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