Recombinant Drosophila melanogaster Serine/threonine-protein phosphatase alpha-2 isoform (Pp1-87B)

Code CSB-YP318305DLU
MSDS
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Source Yeast
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Code CSB-EP318305DLU
MSDS
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Source E.coli
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Code CSB-EP318305DLU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP318305DLU
MSDS
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Source Baculovirus
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Code CSB-MP318305DLU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Pp1-87B
Uniprot No.
Alternative Names
Pp1-87B; CG5650; Serine/threonine-protein phosphatase alpha-2 isoform; EC 3.1.3.16
Species
Drosophila melanogaster (Fruit fly)
Expression Region
1-302
Target Protein Sequence
MGDVMNIDSI ISRLLEVRGA RPGKNVQLSE GEIRGLCLKS REIFLSQPIL LELEAPLKIC GDIHGQYYDL LRLFEYGGFP PESNYLFLGD YVDRGKQSLE TICLLLAYKI KYSENFFLLR GNHECASINR IYGFYDECKR RYSIKLWKTF TDCFNCLPVA AIVDEKIFCC HGGLSPDLTS MEQIRRIMRP TDVPDQGLLC DLLWSDPDKD TMGWGENDRG VSFTFGAEVV AKFLQKHEFD LICRAHQVVE DGYEFFAKRM LVTLFSAPNY CGEFDNAGAM MSVDDTLMCS FQILKPADKR KK
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Is essential for the regulation of mitotic chromosomal segregation as well as regulation of chromatin condensation during interphase.
Gene References into Functions
  1. Ectopic expression of dRif1 causes enlarged larval imaginal discs and early pupal lethality which is completely reversed by co-expression of PP1 87B, the major protein phosphatase in Drosophila, indicating genetic and functional interaction PMID: 26022086
  2. these findings shed light on the in vivo role of the PNUTS-PP1 holoenzyme and its contribution to the control of gene expression during early Drosophila development. PMID: 24204300
  3. PP1-87B has a role in coupling moesin-dependent cell shape changes to mitotic exit. PMID: 22209527
  4. PP1c not only binds TRX, but is a physiologically relevant regulator of TRX function in vivo. PMID: 12524522
Subcellular Location
Cytoplasm.
Protein Families
PPP phosphatase family, PP-1 subfamily
Database Links

KEGG: dme:Dmel_CG5650

STRING: 7227.FBpp0082067

UniGene: Dm.1623

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