Recombinant Drosophila melanogaster Single-stranded DNA-binding protein, mitochondrial (mtSSB)

Code CSB-YP347528DLU
MSDS
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Source Yeast
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Code CSB-EP347528DLU
MSDS
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Source E.coli
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Code CSB-EP347528DLU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP347528DLU
MSDS
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Source Baculovirus
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Code CSB-MP347528DLU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
mtSSB
Uniprot No.
Alternative Names
mtSSB; lopo; CG4337; Single-stranded DNA-binding protein; mitochondrial; Dm mtSSB; Mt-SSB; MtSSB; Protein low power
Species
Drosophila melanogaster (Fruit fly)
Expression Region
23-146
Target Protein Sequence
ATTTTAAA PAKVEKTVNT VTILGRVGAD PQLRGSQEHP VVTFSVATHT NYKYENGDWA QRTDWHRVVV FKPNLRDTVL EYLKKGQRTM VQGKITYGEI TDQQGNQKTS TSIIADDVLF FRDANN
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Binds preferentially and cooperatively to pyrimidine rich single-stranded DNA (ss-DNA). Required to maintain the copy number of mitochondrial DNA (mtDNA) and plays crucial roles during mtDNA replication that stimulate activity of the gamma complex polymerase tam at the replication fork. Promotes tam activity largely by organizing the template DNA and eliminating secondary structures to favor ss-DNA conformations that facilitate tam activity.
Gene References into Functions
  1. The stimulatory effect of mtSSB on Pol gamma on these ssDNA templates is not species-specific. PMID: 26446790
  2. mtSSB uses distinct structural elements to interact functionally with its mtDNA replisome partners and to promote proper mtDNA replication in animal cells PMID: 21953457
Subcellular Location
Mitochondrion.
Tissue Specificity
Uniformly distributed in the early embryo. High levels detected in the anterior and posterior midgut primordia of stage 12 embryos. In larvae, high levels were detected in proliferating tissues including the CNS and digestive tract. In adults, highly expr
Database Links

KEGG: dme:Dmel_CG4337

STRING: 7227.FBpp0099503

UniGene: Dm.5334

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