Recombinant Drosophila melanogaster Spectrin beta chain (beta-Spec), partial

Code CSB-EP251598DLU
MSDS
Size $388
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  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Product Details

Purity
Greater than 95% as determined by SDS-PAGE.
Activity
Not Test
Target Names
beta-Spec
Uniprot No.
Research Area
Others
Species
Drosophila melanogaster (Fruit fly)
Source
E.coli
Expression Region
1-271aa
Target Protein Sequence
MTTDISIVRWDPSQGPGNEYIDEYEYDGGNSSSRLFERSRIKALAEERESVQKKTFTKWVNSHLCRVNCRIADLYVDMRDGKHLIKLLEVLSGERLPKPTKGKMRIHCLENVDKALQFLREQRVHLENIGSHDIVDGNASLNLGLIWTIILRFQIQDITIEEVDNKETKSAKDALLLWCQMKTAGYHNVNVRNFTTSWRDGLAFNAIIHKHRPDLVQFEKLSKTNAIHNLNNAFDVAEDKLGLAKLLDAEDVFVEHPDEKSIITYVVTYYH
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight
35.3
Protein Length
Partial
Tag Info
N-terminal 6xHis-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
Datasheet & COA
Please contact us to get it.

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Target Background

Function
Spectrin is the major constituent of the cytoskeletal network underlying the erythrocyte plasma membrane. It associates with band 4.1 and actin to form the cytoskeletal superstructure of the erythrocyte plasma membrane. Interacts with calmodulin in a calcium-dependent manner.
Gene References into Functions
  1. The abnormal stress fiber-like actin structure on the basal side of follicle cells provides a likely link between the b-spectrin mutations and the loss of the Hippo signaling activity phenotype. PMID: 25589787
  2. Model in which alphabeta-spectrin directly couples lipid uptake at the plasma membrane to lipid droplet growth in the cytoplasm. In contrast, strong overexpression of beta-spectrin caused fat body atrophy and larval lethality. PMID: 24037266
  3. Data show that DAnk2-binding is critical for beta spectrin function in vivo. PMID: 20573981
  4. Spectrin may be an active partner in the stabilization of polarized membrane domains PMID: 14734656
  5. Presynaptic spectrin functions as an essential presynaptic scaffold that may link synaptic cell adhesion with the stabilization of the underlying microtubule cytoskeleton. PMID: 15916948
  6. Brush border spectrin is required for early endosome recycling. PMID: 16537648
  7. At the Drosophila neuromuscular junction postsynaptic Spectrin-actin lattice acts as an organizing scaffold upon which pre- and postsynaptic development are arranged. PMID: 17088429
  8. beta-Spectrin is required in neurons for proper midline axon guidance in the embryonic CNS. PMID: 17121810

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Subcellular Location
Cytoplasm, cytoskeleton. Cytoplasm, cell cortex.
Protein Families
Spectrin family
Database Links

KEGG: dme:Dmel_CG5870

STRING: 7227.FBpp0074228

UniGene: Dm.7022

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