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Recombinant Drosophila melanogaster Steroid receptor seven-up, isoform A (svp), partial

Recombinant Drosophila melanogaster Steroid receptor seven-up, isoform A (svp), partial

Code	CSB-YP321439DLU
MSDS	MSDS
Size	Pls inquire
Source	Yeast
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Code	CSB-EP321439DLU
MSDS	MSDS
Size	Pls inquire
Source	E.coli
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Code	CSB-EP321439DLU-B
MSDS	MSDS
Size	Pls inquire
Source	E.coli
Conjugate	Avi-tag Biotinylated E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code	CSB-BP321439DLU
MSDS	MSDS
Size	Pls inquire
Source	Baculovirus
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Code	CSB-MP321439DLU
MSDS	MSDS
Size	Pls inquire
Source	Mammalian cell
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Product Details
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Customer Reviews and Q&A
Target Background

Product Details

Purity

>85% (SDS-PAGE)

Target Names

svp

Uniprot No.

P16376

Alternative Names

svp; NR2F3; CG11502Steroid receptor seven-up; isoform A; Nuclear receptor subfamily 2 group F member 3; isoform A

Species

Drosophila melanogaster (Fruit fly)

Protein Length

Partial

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function

Receptor that is required in photoreceptors R1, R3, R4 and R6 during eye development; generation of the ganglion mother cell-2 (GMC-2) fate in the nb7-3 lineage, coinciding with the transition in the expression of HB to KR in the neuroblasts (NBs).

Gene References into Functions

Authors propose that the Seven-up-initiated Imp/Syp gradients create coarse temporal windows within type II neuroblasts to pattern INPs, which subsequently undergo fine-tuned subtemporal patterning. PMID: 28434858
A novel allele of svp was isolated. Expression analysis showed that svp is expressed in two distinct pulses in the embryonic NB5-6T lineage. PMID: 22071101
Appropriate recruitment of the R3/R4 photoreceptor pair mediated by svp is a necessary prerequisite for the correct response of the ommatidial precluster to the Fz polarity signal. PMID: 9651479
expression in combination with prospero and elav identifies progenitor cell types during sense-organ differentiation in the Drosophila antenna PMID: 12606283
svp modifies the self-renewal stem cell program to allow chronological change of cell fates, thereby generating neuronal diversity. PMID: 15691762
Results suggest that svp is required for the differentiation of Eve-positive pericardial cells and DA1 muscle founders and may have a role in EPC cell growth PMID: 16450585
Enhancer of svp is initially activated by the NK homeodomain factor Tinman (Tin) via two conserved Tin binding sites. PMID: 17098220
Normal patterning of alary muscles along the dorsal vessel was found to be a function of the Bithorax Complex genes abdominal-A (abd-A) and Ultrabithorax (Ubx) but not of the orphan nuclear receptor gene svp. PMID: 19272319

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Subcellular Location

Nucleus.

Protein Families

Nuclear hormone receptor family, NR2 subfamily

Tissue Specificity

Expressed in several embryonic tissues; dorsal vessel, oenocyte and fat body. CNS expression is dynamic and confined to temporally restricted subsections of the NB lineage; expressed in many NB and GMCs, but only a small number of neurons.

Database Links

STRING: 7227.FBpp0082035

UniGene: Dm.20676

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