Recombinant Drosophila melanogaster Supporter of activation of yellow protein (e (y)3), partial

Code CSB-YP892909DLU
MSDS
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Source Yeast
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Code CSB-EP892909DLU
MSDS
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Source E.coli
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Code CSB-EP892909DLU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP892909DLU
MSDS
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Source Baculovirus
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Code CSB-MP892909DLU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
e(y)3
Uniprot No.
Alternative Names
e(y)3; l(1)G0084; SAYP; CG12238; Supporter of activation of yellow protein; Protein enhancer of yellow 3
Species
Drosophila melanogaster (Fruit fly)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Essential transcription regulator during early development. Coactivates transcription of some euchromatin genes and repress transcription in of euchromatin genes translocated to heterochromatin.
Gene References into Functions
  1. In the repressed state of the ftz-f1 gene, the Pol II complex is pre-recruited on the promoter; Pol II starts transcription but is paused 1.5 kb downstream of the promoter, with SAYP and Brahma forming a nucleosomal barrier ahead of paused Pol II. PMID: 22638575
  2. SAYP interacts with DHR3 nuclear receptor and activates the corresponding genes by recruiting the BTFly (Brahma and TFIID) coactivator supercomplex during development and have multiple common targets across the genome. PMID: 22567850
  3. The transcription coactivators SAYP and ENY2 act in gene expression as cooperative nanoregulators,determining the molecular mechanisms of spatial and temporal coordination of the processes involved in the realization of genetic information. PMID: 22117439
  4. DHR3 and SAYP interact during development and have multiple common targets across the genome PMID: 21519192
  5. Novel conservative domain of SAYP coactivator mediates TFIID and Brahma transcriptional complexes interaction PMID: 21090241
  6. SAYP serves as a basis for assembling the BTFly nuclear supercomplex consising of the Brahma and TFIID coactivators. BTFly may play an important role in ontogenesis. PMID: 20873199
  7. SAYP is a signature subunit that plays a major role in the functional specificity of the PBAP holoenzyme. PMID: 18299390
  8. The existence of a stable coactivator supercomplex consisting of the chromatin-remodeling factor Brahma (SWI/SNF) and the transcription initiation factor TFIID, is shown. PMID: 19541607

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Subcellular Location
Nucleus. Cytoplasm. Chromosome. Note=On polytene chromosomes, it mainly localizes to transcriptionally active euchromatin sites but also localizes to heterochromatic regions such as the chromocenter and the chromosome 4. Cytoplasmic in nursing cells and growing oocytes.
Protein Families
SAYP family
Tissue Specificity
Widely expressed. Highly expressed in ovary. Expressed in nursing cells and growing oocytes at all stages of development and accumulates in mature oocytes. Expressed in the nuclei of syncytium blastoderm of early embryos and in the nuclei of different tis
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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