Recombinant Drosophila melanogaster Transcription factor Ken (ken), partial

Code CSB-YP525658DLU
MSDS
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Source Yeast
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Code CSB-EP525658DLU
MSDS
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Source E.coli
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Code CSB-EP525658DLU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP525658DLU
MSDS
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Source Baculovirus
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Code CSB-MP525658DLU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
ken
Uniprot No.
Alternative Names
ken; CG5575Transcription factor Ken; Protein Ken and Barbie
Species
Drosophila melanogaster (Fruit fly)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Transcription factor required for terminalia development. Negative regulator of the JAK/STAT pathway: represses JAK/STAT-dependent expression of ventral veins lacking (vvl) in the posterior spiracles.
Gene References into Functions
  1. In ken/barbie male flies lacking external genitalia, courtship and physical contact without ejaculation was unable to replicate the mating effect. PMID: 28851647
  2. Data show that that BCL6 homolog ken and barbie (Ken) has an important role in the inhibition of somatic cyst stem cells (CySCs) differentiation. PMID: 22580161
  3. Ken is a novel regulator of JAK/STAT signaling whose dynamic spatial and temporal expression is capable of selectively modulating the transcriptional repertoire elicited by activated STAT92E in vivo. PMID: 16401426
  4. Ken and barbie mutants have defects in escape behaviour, behavioural responses to visual stimuli and synaptic functions in the giant fibre system. PMID: 16879616
Subcellular Location
Nucleus.
Tissue Specificity
Expressed from stage 5 in two rather faint stripes at positions of 64% (anterior domain; AD) and 17% (posterior domain; PD) egg length. During early gastrulation, at stage 6, these two stripes become more evident and detectable at the region posterior to
Database Links

KEGG: dme:Dmel_CG5575

STRING: 7227.FBpp0072038

UniGene: Dm.2792

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