Recombinant Drosophila melanogaster Transcription factor castor (cas), partial

Code CSB-YP748742DLU
MSDS
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Source Yeast
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Code CSB-EP748742DLU
MSDS
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Source E.coli
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Code CSB-EP748742DLU-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP748742DLU
MSDS
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Source Baculovirus
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Code CSB-MP748742DLU
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
cas
Uniprot No.
Alternative Names
cas; ming; CG2102Transcription factor castor; Protein ming
Species
Drosophila melanogaster (Fruit fly)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Transcription factor that specifies expression of key genes in developing central nervous system (CNS). Essential for many, if not all, late developing neuroblastoma (NB) sublineages. Binds to the 5'-[CG]C[CT][CT]AAAAA[AT]-3' DNA sequence, like hb, suggesting that cas and hb act as a late regulators in early and late CNS NB sublineage, respectively. Acts by repressing expression of nub/pdm-1 and pdm2/pdm-2 POU genes, and restrict their pattern of expression in appropriate cells. Required for a full expression of vvl/drifter and acj6/I-POU; it is however unknown whether it directly activates these genes. Controls engrailed (en) expression in the ventral nerve chord.
Gene References into Functions
  1. The expression of castor protein is regulated by ABD-D protein in promoter region of castor gene. PMID: 24219869
  2. Castor (Cas) is a nuclear protein that is expressed in follicle stem cells and early follicle cell precursors and then is restricted to differentiated polar and stalk cells once egg chambers form PMID: 23610413
  3. a novel function for castor in regulating neuropeptide gene expression. PMID: 23010511
  4. These results indicate that cas could be directly suppressed by Repo. PMID: 19635453
Subcellular Location
Nucleus.
Tissue Specificity
Expressed in a specific subset of neuroblasts in the ventral nerve chord and the procephalic region in the embryo. Expressed in many, if not all, late delaminating NBs, and in early NBs, but only after they have undergone several rounds of ganglion mother
Database Links

KEGG: dme:Dmel_CG2102

STRING: 7227.FBpp0078374

UniGene: Dm.1871

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