Recombinant Drosophila melanogaster Transcriptional activator cubitus interruptus (ci), partial

Code CSB-YP323942DLU
MSDS
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Source Yeast
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Code CSB-EP323942DLU
MSDS
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Source E.coli
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Code CSB-EP323942DLU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP323942DLU
MSDS
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Source Baculovirus
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Code CSB-MP323942DLU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
ci
Uniprot No.
Alternative Names
ci; ci-D; CG2125Transcriptional activator cubitus interruptus; Transcriptional activator ci; ci form of 155 kDa; ci-155; ci full-length protein; ciFL) [Cleaved into: Transcriptional repressor cubitus interruptus; Transcriptional repressor ci; ci C-terminally truncated form; ci form of 75 kDa; ci-75)]
Species
Drosophila melanogaster (Fruit fly)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Has a dual function as a transcriptional activator and a repressor of the hedgehog (Hh) pathway. The full-length ci form (ciFL), acts as an activator (ciA) while ciR, its C-terminally truncated form, acts as a repressor. Involved in segment polarity. Required for the normal development of the posterior half of each embryonic segment. Engrailed protein directly represses ci expression in posterior compartment cells. Essential component of a hh-signaling pathway which regulates the Duox-dependent gut immune response to bacterial uracil; required to activate Cad99C-dependent endosome formation, norpA-dependent Ca2+ mobilization and p38 MAPK, which are essential steps in the Duox-dependent production of reactive oxygen species (ROS) in response to intestinal bacterial infection.
Gene References into Functions
  1. Ci influences the Hippo signaling via interaction with Hpo and Yki. PMID: 26403189
  2. Multisite interaction with Sufu regulates Ci/Gli activity through distinct mechanisms in Hh signal transduction. PMID: 25941387
  3. Ci responds to and relays Hh signals in a precise manner to control target genes expressions and ensures proper Hh signal transduction. PMID: 25495033
  4. findings reveal a conserved mechanism by which Ci/Gli is stabilized by a deubiquitination enzyme and identify Usp7/HUASP as a critical regulator of Hh signaling PMID: 26120032
  5. The requirement for gene activation by Ci in the regions of low-to-moderate Hh signalling results in evolutionary pressure favouring weak binding sites in enhancers of certain Hh target genes. PMID: 24218631
  6. CK2 regulated the stability of full-length Cubitus interruptus (Ci). PMID: 20876583
  7. The last 3 zinc fingers of Ci-155 bind Cos2 in vitro. The zinc finger region, rather than the CDN domain, functions redundantly with the CORD domain to promote Hh-regulated Ci-155 proteolysis in wing discs. PMID: 20850429
  8. Proteolysis of the Hedgehog signaling effector Cubitus interruptus requires phosphorylation by Glycogen Synthase Kinase 3 and Casein Kinase 1. PMID: 11955435
  9. ci mediates hedgehog signaling to determine the anteroposterior compartment of Drosophila wing disc. PMID: 12791275
  10. Cos2 recruits multiple kinases to efficiently phosphorylate Ci, and Hh inhibits Ci phosphorylation by specifically interfering with kinase recruitment. PMID: 15691767
  11. Two unrelated transcription factors, Ci and NF-kappaB, use the same mechanism to undergo partial degradation by the proteasome in vivo. PMID: 16299518
  12. Ci proteolysis is compromised, its specific activity is limited principally by Su(fu), and not by Cos2 cytoplasmic tethering or PKA phosphorylation. PMID: 17925225
  13. Results present a model whereby the zinc finger region and lysine 750 together form a unique protection signal that prevents the complete degradation of Cubitus interruptus by the proteasome. PMID: 18625727
  14. Cos2 motility regulates the amounts and activity of Ci that ultimately interpret the level of Hh to which cells are exposed. PMID: 18691888
  15. identified PP4 and PP2A as phosphatases that influence Hh signaling by regulating Smo and Ci, respectively PMID: 19088085
  16. The function of this transcription factor is regulated by a Ci complex that is part of the Hedgehog signaling pathway. PMID: 11476581

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Subcellular Location
Nucleus.
Protein Families
GLI C2H2-type zinc-finger protein family
Database Links

KEGG: dme:Dmel_CG2125

STRING: 7227.FBpp0088245

UniGene: Dm.3166

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