Recombinant Drosophila melanogaster Transient-receptor-potential-like protein (trpl), partial

Code CSB-YP344561DLU
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Source Yeast
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Code CSB-EP344561DLU
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Source E.coli
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Code CSB-EP344561DLU-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP344561DLU
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Source Baculovirus
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Code CSB-MP344561DLU
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
trpl
Uniprot No.
Alternative Names
trpl; CG18345; Transient-receptor-potential-like protein
Species
Drosophila melanogaster (Fruit fly)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
A light-sensitive calcium channel that is required for inositide-mediated Ca(2+) entry in the retina during phospholipase C (PLC)-mediated phototransduction. Required for vision in the dark and in dim light. Binds calmodulin. Trp and trpl act together in the light response, although it is unclear whether as heteromultimers or distinct units. Also forms a functional cation channel with Trpgamma. Activated by fatty acids, metabolic stress, inositols and GTP-binding proteins.
Gene References into Functions
  1. The effect of experimental infection with P. aeruginosa on trpl expression in male Drosophila melanogaster flies from different genetic backgrounds exposed by pricking or by ingestion showed downregulation in the whole body and the intestines. The downregulation of trpl in gammaCop mutant males is not confirmed by qRT-PCR data. PMID: 29616531
  2. effects of cholesterol depletion on the TRPL channel activity PMID: 28863818
  3. CG17352 (CULD) plays an important role in the endocytic turnover of Rh1 and TRPL. PMID: 26598556
  4. conclusion, TTD14 is a novel regulator of TRPL trafficking, involved in internalization and subsequent sorting of TRPL into the recycling pathway that enables this ion channel to return to the plasma membrane PMID: 26509977
  5. The transient receptor potential-like (TRPL) cation channel is a direct target for camphor in gustatory receptor neurons; long-term feeding on a camphor diet leads to reversible downregulation of TRPL protein concentrations. PMID: 24013593
  6. TRP and TRPL assemble exclusively as homomultimeric channels in their native environment. PMID: 23687378
  7. a light-dependent switch in the phosphorylation pattern of the TRPL channel mediates stable expression of TRPL in the rhabdomeres upon prolonged dark-adaptation. PMID: 23592784
  8. These observations show that reduction in TRPC channel function significantly reduces the sensitivity of the olfactory response to CO(2) concentrations of 5% or less in adult Drosophila PMID: 23185459
  9. TRPL channel is constitutively active in S2 cells but is silent in HEK cells. PMID: 22627924
  10. The involvement of de novo protein synthesis in TRPL translocation, was examined. PMID: 22363689
  11. PI(4,5)P(2) is a crucial substrate for PLC-mediated activation of the trpl channels, whereas PUFA may function as the channel activator. PMID: 22065576
  12. the essential function of the TRP channels in TRPL translocation is to enhance Ca(2+)-influx. PMID: 21816824
  13. TRPL translocation represents a novel mechanism to fine tune visual responses. PMID: 11931743
  14. Arrestin2 is required for the rhabdomeric localization and stability of TRPL channels. PMID: 16787936
  15. Whole cell recordings from a Drosophila mutant expressing only TRPL indicated that Ca(2+) block also accounts for the voltage dependence of the native TRPL channels. PMID: 17190901
  16. TRPL and TRP are required for cool avoidance; however they are dispensable for warm avoidance PMID: 18787131
  17. the unitary currents from Drosophila trpl and trp photoreceptors correspond to TRP and TRPL. PMID: 19261713

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Subcellular Location
Membrane; Multi-pass membrane protein. Note=In the dark, there is 20 fold more rhabdomeral trpl protein forming plasma membrane channels than in the light. In the light, the protein translocates to an intracellular compartment. Protein levels remain unchanged in light and dark conditions.
Protein Families
Transient receptor (TC 1.A.4) family, STrpC subfamily
Tissue Specificity
Expressed predominantly in the rhabdomeres of photoreceptor cells.
Database Links

KEGG: dme:Dmel_CG18345

STRING: 7227.FBpp0290876

UniGene: Dm.546

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