Recombinant Drosophila melanogaster Transient receptor potential protein (trp), partial

Code CSB-YP325665DLU
MSDS
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Source Yeast
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Code CSB-EP325665DLU
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Source E.coli
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Code CSB-EP325665DLU-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP325665DLU
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Source Baculovirus
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Code CSB-MP325665DLU
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
trp
Uniprot No.
Alternative Names
trp; CG7875; Transient receptor potential protein
Species
Drosophila melanogaster (Fruit fly)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
A light-sensitive calcium channel that is required for inositide-mediated Ca(2+) entry in the retina during phospholipase C (PLC)-mediated phototransduction. Ca(2+) influx may then feed back and inhibit PLC, thereby facilitating phosphatidylinositol 4,5 bisphosphate (PIP2) recycling. Trp and trpl act together in the light response, though it is unclear whether as heteromultimers or as distinct units, and are activated by fatty acids and metabolic stress. Also required for olfactory adaptation and may be involved in olfactory system development.
Gene References into Functions
  1. Calmodulin binding could induce secondary structure changes in Drosophila TRP C-terminus.TRP 783-862 is a novel calmodulin binding site PMID: 29730291
  2. results indicate that properties of the light-activated channels together with the dynamic light-dependent process of TRP phosphorylation at the S936 site determine response kinetics PMID: 28762890
  3. TRP de-phosphorylation affects the detection limit of oscillating light in photoreceptor cells. PMID: 28314815
  4. Data show that phosphorylation of most ransient receptor potential (TRP) ion channel sites depends on the phototransduction cascade and the activity of the TRP. PMID: 24040070
  5. These results indicate that the Pain TRP channels in the MBs and IPCs control neuronal plasticity that is required for the formation of a certain type of long-lasting associative memory in Drosophila. PMID: 23247253
  6. members of the TRP family are "special assignment" channels, which are recruited to diverse signaling pathways. PMID: 11917094
  7. Single amino acid change in the fifth transmembrane segment of the TRP Ca2+ channel causes massive degeneration of photoreceptors. PMID: 12107168
  8. Paper briefly overviews current knowledge of TRP and its mammalian homologues as non-voltage-gated Ca2+ channels that are constitutively active or gated by a multitude of stimuli; thus TRPs may play divergent roles in cell pathophysiology. PMID: 12719655
  9. Data show that a substantial reduction of the TRP channel protein by RNA interference completely rescues neuronal degeneration and significantly improves the light-elicited responses of the eye. PMID: 15342118
  10. TRP has independent channel and scaffolding roles PMID: 16301334

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Subcellular Location
Cell membrane; Multi-pass membrane protein. Note=Localized on plasma membrane loops found at the base of the rhabdomere, in close proximity to the calcium stores.
Protein Families
Transient receptor (TC 1.A.4) family, STrpC subfamily
Tissue Specificity
Expressed predominantly in the rhabdomeres of photoreceptor cells. Expressed in the third antennal segment and in the olfactory segment at approximately 70 hours after puparium formation during antennal development.
Database Links

KEGG: dme:Dmel_CG7875

STRING: 7227.FBpp0084879

UniGene: Dm.21694

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