Recombinant Drosophila melanogaster Tyrosine-protein kinase Drl (drl), partial

Code CSB-YP630635DLU
MSDS
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Source Yeast
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Code CSB-EP630635DLU
MSDS
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Source E.coli
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Code CSB-EP630635DLU-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP630635DLU
MSDS
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Source Baculovirus
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Code CSB-MP630635DLU
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
drl
Uniprot No.
Alternative Names
drl; lio; CG17348Tyrosine-protein kinase Drl; EC 2.7.10.1; Protein derailed
Species
Drosophila melanogaster (Fruit fly)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Probable coreceptor of Wnt proteins. Involved in neuronal pathway recognition and ventral muscle attachment site selection. Non-vital for development. May be part of a signal transduction cascade involved in learning and possibly memory.
Gene References into Functions
  1. Drl promotes dorsolateral targeting of projection neuron dendrites in developing antennal lobe. PMID: 25378162
  2. Homodimerization of the Wnt receptor DERAILED recruits the Src family kinase SRC64B in the developing embryonic central nervous system. PMID: 23979591
  3. Role of the cytoplasmic domain and the kinase activity of the linotte/derailed receptor tyrosine kinase in adult brain development. PMID: 15611849
  4. Both the Wnt4-frizzled pathway and a separate Wnt5-derailed pathway are needed for proper salivary gland migration in Drosophila embryogenesis. PMID: 17507403
  5. Wnt5 regulates the growth of the Drosophila neuromuscular junction (NMJ) by signaling through the Derailed receptor. Mutations in both wnt5 and drl result in a significant reduction in the number of synaptic boutons. PMID: 17963254
  6. Derailed protein receptor (Drl) is localized in developing olfactory glomeruli and mediates Wnt5 signaling. PMID: 19369566

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Subcellular Location
Cell membrane; Single-pass membrane protein.
Protein Families
Protein kinase superfamily, Tyr protein kinase family
Tissue Specificity
In the embryonic abdominal hemisegment, expression is restricted to cell body, axon and growth cone of a cluster of 20 ventral nerve cord interneurons. During muscle growth and attachment events in the embryonic abdominal hemisegment, expression is in som
Database Links

KEGG: dme:Dmel_CG17348

STRING: 7227.FBpp0303242

UniGene: Dm.4108

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