Recombinant Drosophila melanogaster Vacuolar protein-sorting-associated protein 36 (Vps36)

Code CSB-YP892898DLU
MSDS
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Source Yeast
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Code CSB-EP892898DLU
MSDS
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Source E.coli
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Code CSB-EP892898DLU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP892898DLU
MSDS
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Source Baculovirus
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Code CSB-MP892898DLU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Vps36
Uniprot No.
Alternative Names
Vps36; CG10711Vacuolar protein-sorting-associated protein 36; ESCRT-II complex subunit VPS36
Species
Drosophila melanogaster (Fruit fly)
Expression Region
1-399
Target Protein Sequence
MNRFAYVEAR LSPNESFVSR DNRVKIYDGD QKTDFEDGEV VLTTHRLFWG RPGEIARAAV TLCLPLSYVI SVSEETTASN FFGRKTRIIM HLHPPTSDKG PGPLDTSRAT HIKLSGKNGL SVEFHSALRE TLNARVWEIL LTSEIIINGV ASSPTLEPAN DRLARIQKRT GIGGIERHLE AKAKATDENI ALAFQDLSVL MAMAKDMVGV SKTISSKIRK QKGEISDDET VRFKSYLMSL GIDDPVTRDN FTSNSAYFSS LARQICEMLL DPIEEQGGMM SLADVYCRVN RARGLELLSP EDLLHACEQL SGPIRLRSFP SGARVLQLES HDDALIAVDT LEKVEAAESL AVEELAKQLG ISLLLAKERL LVAERLGKVC RDESVEGLRF YPNLLLGRD
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Component of the ESCRT-II complex (endosomal sorting complex required for transport II), which is required for multivesicular body (MVB) formation and sorting of endosomal cargo proteins into MVBs. The MVB pathway mediates delivery of transmembrane proteins into the lumen of the lysosome for degradation. The ESCRT-II complex is probably involved in the recruitment of the ESCRT-III complex. ESCRT-II interacts, through the Vps36 subunit, with bicoid mRNA. This interaction is required for the anterior localization of bicoid mRNA in the developing egg. Plays a role in promoting mitochondrial autophagy (mitophagy).
Gene References into Functions
  1. vps36 mutations increase apoptotic resistance, but have little effect on non-autonomous proliferation. PMID: 19132102
Subcellular Location
Cytoplasm.
Protein Families
VPS36 family
Tissue Specificity
Ubiquitously expressed in the ovary.
Database Links

KEGG: dme:Dmel_CG10711

STRING: 7227.FBpp0075560

UniGene: Dm.7252

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