Recombinant Drosophila melanogaster Voltage-dependent calcium channel type A subunit alpha-1 (cac), partial

Code CSB-YP310084DLU
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Source Yeast
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Code CSB-EP310084DLU
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Source E.coli
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Code CSB-EP310084DLU-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP310084DLU
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Source Baculovirus
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Code CSB-MP310084DLU
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
cac
Uniprot No.
Alternative Names
cac; nbA; nonB; CG43368; Voltage-dependent calcium channel type A subunit alpha-1; Protein cacophony; Protein nightblind A; Protein no-on-transient B; Dmca1A
Species
Drosophila melanogaster (Fruit fly)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Voltage-sensitive calcium channels (VSCC) mediate the entry of calcium ions into excitable cells and are also involved in a variety of calcium-dependent processes, including muscle contraction, neurotransmitter release, gene expression, cell motility, cell division and cell death. Probably encodes a dihydropyridine-insensitive current. Vital for survival to adulthood.
Gene References into Functions
  1. Motor defects in TDP-43 mutants can be rescued by expression of cacophony in motor neurons and by expression in two pairs of neurons in the brain. PMID: 28847811
  2. the novel R1673P allele of CACNA1A produces neurodegenerative phenotypes in flies and human, likely due to a toxic gain of function PMID: 28742085
  3. cacophony (cacTS2), a mutation of the Drosophila presynaptic Ca++ channel alpha1 subunit gene, is a particularly potent seizure-suppressor mutation, reverting seizure-like phenotypes for parabss1 and other BS mutants PMID: 26771829
  4. Cac regulates lysosomal fusion with endosomes and autophagosomes and is required for neuronal homeostasis. PMID: 25811491
  5. Homeostatic depression is achieved by decreased presynaptic Cav2.1 calcium channel abundance and calcium influx. PMID: 25884248
  6. Dopaminergic neurons with mutant Ca2+ channel cacophony are inherently resistant to MPP+-induced neurodegeneration. PMID: 25445363
  7. cac and Dmca1D Ca(2+) channels differentially contribute to functional and structural aspects of slo-induced synaptic modifications PMID: 23959639
  8. Vesicle exocytosis depends on cacophony, TRP and TRPL. PMID: 22952921
  9. Homeostatic increase in Ca(2+) influx and release is blocked by a point mutation in the presynaptic CaV2.1 channel. PMID: 22633807
  10. role of mutation in courtship and other behaviors PMID: 12242229
  11. Dmca1A is an allele of the N-type calcium channel locus that caused synaptic undergrowth PMID: 12896973
  12. Localization of cacophony on presynaptic terminals at neuromuscular synapses. PMID: 14715960
  13. Data show that the Drosophila cacophony ts2 mutation reduces presynaptic Ca2+ entry and defines an important element in Cav2.1 channel inactivation PMID: 16820014
  14. These data confirm the presynaptic expression of synaptic homeostasis and implicate presynaptic Ca(V)2.1 in a homeostatic retrograde signaling system. PMID: 17114050
  15. Cacophony protein plays a key role in developmental regulation of Drosophila neuronal excitability. PMID: 17267561
  16. Neuronal overexpression of cac partially rescues the viability and physiological defects in stj mutants, indicating a role for the alpha(2)delta Ca(2+) channel subunit in mediating the proper localization of an alpha(1) subunit at synapses. PMID: 18391075
  17. cac encoded Ca(v)2-type calcium channels regulate action potential (AP)-independent release of neurotransmitter at excitatory cholinergic synapses PMID: 19004991

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Subcellular Location
Membrane; Multi-pass membrane protein.
Protein Families
Calcium channel alpha-1 subunit (TC 1.A.1.11) family
Tissue Specificity
Expressed widely in the embryonic nervous system.
Database Links

KEGG: dme:Dmel_CG43368

STRING: 7227.FBpp0298326

UniGene: Dm.2395

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