Recombinant Drosophila melanogaster Zygotic gap protein knirps (kni)

Code CSB-YP320787DLU
MSDS
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Source Yeast
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Code CSB-EP320787DLU
MSDS
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Source E.coli
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Code CSB-EP320787DLU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP320787DLU
MSDS
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Source Baculovirus
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Code CSB-MP320787DLU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
kni
Uniprot No.
Alternative Names
kni; NR0A1; CG4717Zygotic gap protein knirps; Nuclear receptor subfamily 0 group A member 1
Species
Drosophila melanogaster (Fruit fly)
Expression Region
1-429
Target Protein Sequence
MNQTCKVCGE PAAGFHFGAF TCEGCKSFFG RSYNNISTIS ECKNEGKCII DKKNRTTCKA CRLRKCYNVG MSKGGSRYGR RSNWFKIHCL LQEHEQAAAA AGKAPPLAGG VSVGGAPSAS SPVGSPHTPG FGDMAAHLHH HHQQQQQQQV PRHPHMPLLG YPSYLSDPSA ALPFFSMMGG VPHQSPFQLP PHLLFPGYHA SAAAAAASAA DAAYRQEMYK HRQSVDSVES QNRFSPASQP PVVQPTSSAR QSPIDVCLEE DVHSVHSHQS SASLLHPIAI RATPTTPTSS SPLSFAAKMQ SLSPVSVCSI GGETTSVVPV HPPTVSAQEG PMDLSMKTSR SSVHSFNDSG SEDQEVEVAP RRKFYQLEAE CLTTSSSSSS HSAAHSPNTT TAHAEVKRQK LGGAEATHFG GFAVAHNAAS AMRGIFVCV
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Transcriptional repressor. Binds to multiple sites in the eve stripe 3 enhancer element. Plays an essential role in the segmentation process both by refining the expression patterns of gap genes and by establishing pair-rules stripes of gene expression.
Gene References into Functions
  1. Phenotypic analysis of these 'in locus' mutations based on both expression of Kni protein and adult wing phenotypes, reveals novel unexpected features of L2-cis-regulatory module function including evidence for a chromosome pairing-dependent process that promotes transcription. PMID: 29274230
  2. Vvl and Kni directly regulate ecdysone biosynthesis by transcriptional control of biosynthetic gene expression and indirectly by affecting prothoracicotropic hormone and TOR/S6 Kinase signaling. PMID: 24945799
  3. Knirps causes local changes of histone density and acetylation, and the inhibition of activator recruitment, without affecting the recruitment of basal transcriptional machinery. PMID: 21353562
  4. identifed a 1.4 kb cis-acting enhancer element from the kni locus that faithfully directs gene expression in the second longitudinal wing vein (L2) primordium PMID: 12466192
  5. absence of maternal DSP1 results in A4 segmentation defects that are correlated with a diminution of the kni expression domain PMID: 12820172
  6. Functional similarity of Knirps CtBP-dependent and C-terminal Binding Protein-independent transcriptional repressor activities. PMID: 12888527
  7. Knirps protein is a short-range transcriptional repressor that locally inhibits activators by recruiting the CtBP co-repressor. PMID: 15128671
  8. DNA-binding Gal4-CtBP fusion proteins also require NAD binding for activity, indicating that NAD plays a role in repression at a step subsequent to CtBP recruitment to the promoter PMID: 15199149

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Subcellular Location
Nucleus.
Protein Families
Nuclear hormone receptor family, NR0 subfamily
Database Links

KEGG: dme:Dmel_CG4717

STRING: 7227.FBpp0077941

UniGene: Dm.2511

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