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Recombinant Enterobacteria phage T4 Protein Gp59 (59)

Code CSB-YP319530EDZ
MSDS
MSDS
Size Pls inquire
Source Yeast
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Code CSB-EP319530EDZ
MSDS
MSDS
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Source E.coli
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Code CSB-EP319530EDZ-B
MSDS
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP319530EDZ
MSDS
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP319530EDZ
MSDS
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
59
Uniprot No.
P13342
Alternative Names
59; Protein Gp59
Species
Enterobacteria phage T4 (Bacteriophage T4)
Expression Region
1-217
Target Protein Sequence
MIKLRMPAGG ERYIDGKSVY KLYLMIKQHM NGKYDVIKYN WCMRVSDAAY QKRRDKYFFQ KLSEKYKLKE LALIFISNLV ANQDAWIGDI SDADALVFYR EYIGRLKQIK FKFEEDIRNI YYFSKKVEVS AFKEIFEYNP KVQSSYIFKL LQSNIISFET FILLDSFLNI IDKHDEQTDN LVWNNYSIKL KAYRKILNID SQKAKNVFIE TVKSCKY
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Protein FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Related Products

59 antibodies

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Target Background

Function
DNA helicase loader protein that participates in viral DNA replication, recombination, and repair. At the fork, required for loading of the replicative helicase onto DNA protected by the ssDNA-binding protein. Coordinates simultaneous synthesis of leading- and lagging-strands.
Gene References into Functions
  1. Results examine the interactions of wild type and mutant forms of the gp41 helicase with the T4 gp59 helicase loader and gp32 single-stranded DNA binding proteins. PMID: 15740739
  2. Bacteriophage T4 helicase loader protein gp59 functions as gatekeeper in origin-dependent replication in vivo PMID: 15781450
  3. Gp59 acts as the helicase clamp loader while forming a gp59-gp43 complex at a DNA fork in the presence of the single-stranded DNA binding protein (gp32). PMID: 15909989
Database Links

KEGG: vg:1258623

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