Home
Protein
Recombinant Protein
Recombinant Enterobacteria phage T7 Bacterial RNA polymerase inhibitor (2)

Recombinant Enterobacteria phage T7 Bacterial RNA polymerase inhibitor (2)

Code	CSB-YP356123EEB
MSDS	MSDS
Size	Pls inquire
Source	Yeast
Have Questions?	Leave a Message or Start an on-line Chat

Code	CSB-EP356123EEB
MSDS	MSDS
Size	Pls inquire
Source	E.coli
Have Questions?	Leave a Message or Start an on-line Chat

Code	CSB-EP356123EEB-B
MSDS	MSDS
Size	Pls inquire
Source	E.coli
Conjugate	Avi-tag Biotinylated E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
Have Questions?	Leave a Message or Start an on-line Chat

Code	CSB-BP356123EEB
MSDS	MSDS
Size	Pls inquire
Source	Baculovirus
Have Questions?	Leave a Message or Start an on-line Chat

Code	CSB-MP356123EEB
MSDS	MSDS
Size	Pls inquire
Source	Mammalian cell
Have Questions?	Leave a Message or Start an on-line Chat

Product Details
Related Products
Customer Reviews and Q&A
Target Background

Product Details

Purity

>85% (SDS-PAGE)

Target Names

Uniprot No.

P03704

Alternative Names

2; Bacterial RNA polymerase inhibitor; Gene product 2; Gp2

Species

Enterobacteria phage T7 (Bacteriophage T7)

Expression Region

1-64

Target Protein Sequence

MSNVNTGSLS VDNKKFWATV ESSEHSFEVP IYAETLDEAL ELAEWQYVPA GFEVTRVRPC VAPK

Protein Length

full length protein

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Customer Reviews and Q&A

■ Customer Reviews

There are currently no reviews for this product.

Submit a Review here

Target Background

Function

Acts as an inhibitor of bacterial RNA polymerase by interacting with RpoC subunit and thus preventing promoter recognition by RpoE. Non-DNA-binding transcription factor that plays a role in the transcriptional switch that abolishes transcription of the viral early genes (class I) by the host RNA polymerase but allows transcription of late genes (class II and class III) by T7 RNA polymerase.

Gene References into Functions

Overexpression of recombinant inhibitor of host bacterial RNA polymerase (Gp2) in exponentially growing E. coli induces changes in RNAP behavior at single molecule level and induces global alterations in the transcriptional programme. PMID: 28977482
gp15 and gp16 can refold from a partially denatured state in vitro, and that gp15 interacts with gp16 to form a spiral ring structure. PMID: 26476287
E24K and F27Y mutations of Gp2 facilitate the interaction between Gp2beta and Escherichia coli RNA polymerase. PMID: 22977089
Gp2 inhibits RPo formation not just by steric occlusion of the RNA polymerase (RNAp)-DNA interaction but also through long-range antagonistic effects on RNAp-promoter interactions around the RNAp active center that likely occur due to repositioning of the amino-terminal sigma(70) domain region 1.1 (R1.1) by Gp2. PMID: 22819324
comprehensive mutagenesis data reveal that two conserved arginine residues located on the opposite side of Gp2 are important for binding to and inhibition of RNAP PMID: 20133868

Hide All

Database Links

KEGG: vg:1261073

Promotions