2 Antibody

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Code	CSB-PA356123XA01EEB
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Target Background

Product Details

Full Product Name

Rabbit anti-Enterobacteria phage T7 (Bacteriophage T7) 2 Polyclonal antibody

Uniprot No.

P03704

Target Names

Alternative Names

2 antibody; Bacterial RNA polymerase inhibitor antibody; Gene product 2 antibody; Gp2 antibody

Raised in

Rabbit

Species Reactivity

Enterobacteria phage T7 (Bacteriophage T7)

Immunogen

Recombinant Enterobacteria phage T7 (Bacteriophage T7) 2 protein

Immunogen Species

Enterobacteria phage T7 (Bacteriophage T7)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

■ Customer Reviews

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Target Background

Function

Acts as an inhibitor of bacterial RNA polymerase by interacting with RpoC subunit and thus preventing promoter recognition by RpoE. Non-DNA-binding transcription factor that plays a role in the transcriptional switch that abolishes transcription of the viral early genes (class I) by the host RNA polymerase but allows transcription of late genes (class II and class III) by T7 RNA polymerase.

Gene References into Functions

Overexpression of recombinant inhibitor of host bacterial RNA polymerase (Gp2) in exponentially growing E. coli induces changes in RNAP behavior at single molecule level and induces global alterations in the transcriptional programme. PMID: 28977482
gp15 and gp16 can refold from a partially denatured state in vitro, and that gp15 interacts with gp16 to form a spiral ring structure. PMID: 26476287
E24K and F27Y mutations of Gp2 facilitate the interaction between Gp2beta and Escherichia coli RNA polymerase. PMID: 22977089
Gp2 inhibits RPo formation not just by steric occlusion of the RNA polymerase (RNAp)-DNA interaction but also through long-range antagonistic effects on RNAp-promoter interactions around the RNAp active center that likely occur due to repositioning of the amino-terminal sigma(70) domain region 1.1 (R1.1) by Gp2. PMID: 22819324
comprehensive mutagenesis data reveal that two conserved arginine residues located on the opposite side of Gp2 are important for binding to and inhibition of RNAP PMID: 20133868

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Database Links

KEGG: vg:1261073

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2 Antibody

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